利用单分子实时测序技术评价原料奶、超高温奶和婴幼儿配方奶粉中的细菌污染.pdfVIP

J. Dairy Sci. 98:1–9 /10.3168/jds.2015-9886 © American Dairy Science Association® , 2015. Evaluation of bacterial contamination in raw milk, ultra-high temperature milk and infant formula using single molecule, real-time sequencing technology 1 1 Qiangchuan Hou, Haiyan Xu, Yi Zheng, Xiaoxia Xi, Lai-Yu Kwok, Zhihong Sun, Heping Zhang, and Wenyi Zhang2 Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, China, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China ABSTRACT try is anticipated to continue into the future (Fuller et al., 2007). Raw milk, UHT milk, and infant formula The Pacific Biosciences (Menlo Park, CA) single are the main types of dairy products consumed by molecule, real-time sequencing technology (SMRT) adults and infants. These dairy products are known to was reported to have some advantages in analyzing contain rich nutrient contents, including carbohydrates, the bacterial profile of environmental samples. In this proteins, and minerals, which may promote the growth study, the presence of bacterial contaminants in raw of microbes as well as some food-borne pathogens (Re- milk, UHT milk, and infant formula was determined menant et al., 2015). The consumption of pathogen- by SMRT sequencing of the full length 16S rRNA gene. containing products may cause illnesses ranging from The bacterial profiles obtained at different taxonomic upset stomach to more serious symptoms (e.g., diar- levels revealed clear differences in bacterial community rhea, fever, vomiting, and so on; Ahmed et al., 2014). str