五氯酚对斑马鱼胚胎毒性效应研究.pdf

应 用 生 态 学 报 2005年 10 月 第 16 卷 第 10 期 CHI ESE JOUR AL OF APPLIED ECOLOGY, Oct.2005, 16( 10)B 1967~ 1971 * 郑 敏 朱 琳* * ( 南开大学环境科学与工程学院, 天津 300071) = 采用斑马鱼胚胎发育技术, 对环境激素类物质五 酚的毒性进行测定, 结果表明, 五 酚(PCP) 对胚胎的特定作用时间段是卵产出至发育6 h 之内; PCP 对胚胎发育有明显的抑制作用, 会造成胚胎发育 的畸形或死亡, 不同时间染毒产生的可观察毒理学终点各异; 随着PCP 对发育 48 h 斑马鱼胚胎作用时间 - 1 的减短,其致死效应敏感性降低, 其中0 hpf 组的LC 值最小, 为 7018 L g# L , 24 hpf 组 LC 值最大, 为 50 50 - 1 8311 8 Lg# L ;斑马鱼胚胎对孵化后 0 时染毒的PCP 最为敏感, PCP 对胚胎产生急性毒性效应的敏感指 标:心胞囊肿、血液循环障碍、无心律 孵化率降低 停滞发育作用; 斑马鱼胚胎最敏感的指标为48 h 血 液循环障碍和48 h 半致死效应. 五 酚 斑马鱼胚胎 毒性效应 1001- 9332(2005) 10- 1967- 05 X502 A Toxicity effects of pentachlorophenol on Brachydanio rerio. ZHE GMin,ZHU Lin( Collegeof Environmental Science and Engineering,Nankai Univer sity, Tianj in 300071, China) .2Chin .J .A l .Ecol .,2005, 16(10):1967~ 1971. With embryo development technique, this paper determined the toxicity of pentachlorophenol (PCP) to Brachy2 danio rerio embryos. Theresults showed that the special effect duration ofPCP on embryos was within 6 hours after embryos incubation.PCP could markedly inhibit the development of Brachydanio rerio embryos, and cause its malformation and even death. There were different toxicity endpoints which could be observed in embryos ex2 posed to PCP with different time. The lethal effect sensitivity of embryos incubated after 48 hours was getting