分光光度法测定畜禽骨素中胶原蛋白含量.pdf

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.10 分光光度法测定畜禽骨素中胶原蛋白含量 董宪兵，李侠，张春晖，孙红梅，李银 （中国农业科学院农产品加工研究所/农业部农产品加工重点实验室，北京 100193） 摘要：本文提供了一种快速、简单、准确、成本低的畜禽骨素中胶原蛋白含量的测定方法。通过热法和酸法提取畜禽骨中胶原 蛋白，计算其中胶原蛋白与羟脯氨酸含量的比值，得到不同原料骨的胶原蛋白换算系数：猪骨为 6.12~6.17，牛骨为 6.77，鸡骨为 6.27~6.30，羊骨为6.34~6.35，结果表明水溶性和酸溶性的胶原蛋白换算系数差异不显著（P0.05 ）。根据骨素生产实际情况,选用水溶 性胶原蛋白换算系数作为骨素中胶原蛋白测定的换算系数，用分光光度法测定热提骨素中羟脯氨酸含量，乘以对应的胶原蛋白换算系 数，得到猪骨素、牛骨素和鸡骨素中胶原蛋白含量分别为9.19%、7.92%、9.78% 。该方法测定骨素中胶原蛋白含量的相对标准偏差在 0.0 1~0.03%之间，变异系数在0.44~2.26%之间，平均回收率在99.36~100.44%之间，说明分光光度法适用于骨素中胶原蛋白含量的测 定。 关键词：分光光度法；畜禽骨素；胶原蛋白换算系数；羟脯氨酸；胶原蛋白 文章篇号：1673-9078(2013)10-2538-2541 Determination of Collagen Content in Livestock and Poultry Ossein by Spectrophotometry DONG Xian-bing, LI Xia, ZHANG Chun-hui, SUN Hong-mei, LI Yin (Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Science/Key Laboratory of Agricultural Product Processing, Ministry of Agriculture, Beijing 100193 , China) Abstract: The paper provided a rapid, simple, accurate and low cost method to determine collagen content in ossein. Collagens from livestock and poultry bone were extracted by acid and thermal processes, and then calculated the ratio of collagen protein and hydroxyproline content in order to obtain collagen conversion factors from different bones. However the results indicat ed that fewer differences of collagen conversion factors between water-soluble and acid soluble collagen could be seen. According to the actual production, water-soluble collagen was taken as the conversion factor in ossein. The hydroxyproline in ossein was measured by spectrophotometry and then the collagen contents of pig, bovine and chicken ossein were calculated as 9.19%, 7.92% and 9.78%, respectively . The relative standard deviation (RSD) w