Colorimetric allele typing through cooperative binding of DNA probes carrying a metal chelator for luminescent lanthanide ions外文翻译.pdfVIP

ANALYTICAL BIOCHEMISTRY Analytical Biochemistry 359 (2006) 259–261 /locate/yabio Notes Tips Colorimetric allele typing through cooperative binding of DNA probes carrying a metal chelator for luminescent lanthanide ions Yusuke Kitamura a, Toshihiro Ihara a,b,*, Yusuke Tsujimura a, Yuka Osawa a Masato Tazaki c, Akinori Jyo a a Department of Applied Chemistry and Biochemistry, Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan b PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan c General Education, Faculty of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan Received 24 June 2006 Available online 5 October 2006 Single nucleotide polymorphisms (SNPs)1 are the most as dehydration/capturing and sensitizer units for Ln3+, common type of genetic variation. They can be used as respectively [8,9]. markers to identify the genes that underlie certain diseases We demonstrated this approach in the analysis of the [1]. For effective screening of a large number of SNPs, we thiopurine S-m