小体鲟AR基因克隆_序列及其组织mRNA表达要点分析.pdfVIP

2014 11 , 21(6): 11461153 Journal of Fishery Sciences of China DOI: 10.3724/SP.J.1118.2014.01146 AR mRNA 1, 2 1 1 3 3 3 1 , , , , , , 1. , 150070; 2. , 201306; 3. , 100039 : RT-PCR RACE , (Acipenser ruthenus )(AR )cDNA , real-time qPCR AR mRNA , AR cDNA 2 953 bp, 5′68 bp (untranslation region, UTR), 2 528 bp ORF(open reading frame)3′327 bp (ploy A ) AR 843 , 94.24 kD , AR AR , (A. ruthenus × Huso huso) (A. baerii )(Oncorhynchus mykiss)(Anguilla japonica )99%98% 83%83%real-time qPCR , AR , , AR , : ; ; ; ; : S917 : A : 10058737(2014)06114608 (Acipenseriformes) , , , “”, , , , [1] (Acipenser ruthenus ) [4], (Acipenseridae), (Acipenserinae), (Acipenser ), , [6–10], [2] , , 0.2 ~7 /kg, [5](Onchorynchus mykiss) , , , [3] [11] (androgen receptor, AR ) :2013-12-09; : 2014-02-27. :() (201003055); (ZD-2012-345);