16种常见致病菌16SrRNA区域进化树的建立.pdfVIP

208 　J Chi M ed U iv　 34　 3　2005 6 16 16S rRNA △ 康素明 ,潘忠诚,张玉魁, 何群,马佳明, 赵雨杰 (, 110001) 　　【】:通过建立 16种常见致病菌的进化树, 了 细菌之间的进化关系, 为设计其诊断用芯片的特异 探针提供理论基础。:常规方法提取细菌DNA, PCR 对称扩增后进行 DNA 测序, 利用 BLAST 和 C lustalW 程序 分析测序结果并建立进化树。:20种细菌中, 16种成功扩增出PCR产物, 得到了理想的测序结果, 同时建立了 这 16种细菌的进化树。:根据进化树所示, 可以设计不同细菌的特异诊断用探针, 利用芯片快速鉴定细菌。 【】细菌;进化树;诊断芯片 【】Q811.4　　　【】A　　　【】0258 -4646(2005)03 - 0208 - 02 Phyligenetic analysis on the 16S rRNA region of16 common nosogenetic bacteria KANG Su-m i g, PAN Zho g-che g, ZHANG Yu-ku,i HE Qu , MA Jia-m i g, ZHAO Yu-jie (Ce ter of Biochip, Co llege of BasicM edical Scie ces, Chi aM edicalU iversity, She ya g 110001, Chi a) 【Abstract】　Objective:To u dersta d the phylige etic relatio ship bewt ee differe t bacteria by establish i g the phylige etic tree of 16 comm o osoge etic bacteria, a d to provide theoretical basis for desig i g special probes i d iag- osticm icroarray for bacteria. M ethods:Routi em ethod was used to extract the bacteriaDNA. TheDNA seque ci gw as perform ed after the polym erase chai reactio (PCR). By usi g the BLAST a d C lustalW program, the seque ci g resu lts w ere a alyzed a d the phylige etic tree w as estab lished. Results:Amo g the 20 k i ds of bacteria, we obtai ed successfu l PCR results for 16 k i ds of bacteria. The seque ci g results a d the phylige etic tree w ere idea.l Conclusion:Based o the phylige etic a alysis, special probes for differe tiati g bacteria ca be desig ed successfully. It is viab le to ide tifyi g differe t bacteria through DNA m icroarray. 【Key ords】　bacteria;phylige etic tree;diag ostic m icroarray 　　(phylige etic tree), 。, - , - DNA, PCR 。 20 , :1=, 2= [1] , 3=, 4=D, 5= 。 2004