麻疯树新基因JcPIP的分子克隆和功能研究.PDFVIP

J cP IP 1, 2 1 1 1 *1 张颖 江璐玎 王云霄 牛蓓 陈放 ( 1() 2) : 从大戟科耐旱植物麻疯树 cDNA 中克隆得到了一个麻疯树 膜内膜蛋白( PIP)新基因(JcPIP ) 其 cRNA 在非洲爪蟾卵母细胞(X enop us oocytes ) 中异源表达后发现细胞膨胀率扩大了 10 倍聚类分析表明, 麻疯树 PIP蛋 白与蓖麻葡萄和菠菜 PIP蛋白在进化上有最近的亲缘关系半定量 RT-PCR研究表明该基因在植株的整 个生长 发育期都有表达但干旱胁迫下 J cPIP 基因表达量没有显著变化 : 水通道蛋白; 功能分析; 干旱胁迫 Cloning and Function of aNew Aquaporin Gene(J cP IP ) from Ja trop ha CurcasM ZHANG Y ing, JIANG Lu- ing,WANG Yun-xiao, NIU Bei, CHEN Fang Abstract: Amp lifie by PCR w ith egenerate prmi ers, a new plasm a membrane intrinsic protein gene (JcP IP ) w as clone from the cDNA of Jatrop ha curcas. The cRNA of JcP IP w as hetero express ion inX enop us oocytes as aw ater channel an in- crease sw elling rate 10 tmi es higher. Phylogen ic analysis in icate that PIPs ofJatrop ha curcas was relation to R icinus com- m unis, Vitis v infi era an Sp inacia oleracea closely. Sem i-quantitative PCR analysis show e itw as expresse in each phase of J atrop ha curcas grow th. Furtherm ore, there were no obviously changes in PIPs expression levels un er rought stress. K ey words:Aquaporin; Function analysis; Drought stress First author. s address:K ey Laboratory of B io-resources an Eco-environm en t, M inistry of E ucation, College of Life Sc-i ence, S ichuan University, 610064, Cheng u, Ch ina. ( Aquaporin) , , [ 1] , 1. 2 RNA提取 [ 2] (J atrop ha curcas) RNA , 1. 3 PCR RT-PCR , , RT-PCR TaK aRaAMV , , , PCR RT-PCR ,