清肺化痰汤对COPD模型大鼠氧化应激的影响.pdfVIP

Qingfei Huatan Decoction on rat COPD model the effects of oxidative stress ABSTRACT Objective: Using endotracheal injection of lipopolysaccharide (LPS) plus smoked cigarettes joint building method to establish the COPD rats model, to give Qingfei Huatan Decoction intervene, detect serum of rats after the intervention in Chinese super oxide dismutase (SOD) activity and reduced glutathione (GSH) and malondialdehyde (MDA) content. Qingfei Huatan Decoction from the Angle of oxidative stress to explore the influence of the rat COPD model, for Qingfei Huatan Decoction in COPD provide experimental basis for clinical applications. Methods: 60 healthy SPF Wistar rats, male, age 7 weeks, weight (230 ±20) g, were randomly divided into A 、B 、C、D 、E, A total of five groups, each group of 12, as the blank group, group A group B for COPD group, group C for n-acetyl cysteine intervention group, low dose group D for Qingfei Huatan Decoction soup group, the group E for Qingfei Huatan Decoction high dose group. Group A normal breeding, dont do special processing, and gives the 29th day 0.9% saline 10 ml/(kg•d) to fill the stomach. B、C、D 、E group respectively in the experiments of day 1 and 14 days the bullishness of tube injection of LPS and 2-13 days and 15 to 28 days smoked cold air stimulation preparation of COPD rats model, a total of 28 days. (29 days) after 28 d, B 、C、D 、E group random executed two rats in the lower right lung tissue pathology observation, judgment made success. After the success of the building, B group was given 0.9% saline 10 ml/ (kg•d) to fill the stomach, C group was given acetylcysteine granules 1 ml/ (kg•d) to fill the stomac