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车前草穗枯病菌分子鉴定
生 物 灾 害 科 学 2012, 35(3): 258 -260
Biological Disaster Science, Vol. 35, No. 3, 2012 swzhkx@163.com
DOI :10.3969/j.issn.2095-3704.2012.03.006
车前草穗枯病菌分子鉴定
1 2 1 1*
梁 艳 ,涂怀妹,崔朝宇,蒋军喜
(1. 江西农业大学 农学院,江西 南昌 330045 ;2. 江西省安义县农业局,江西 安义 330500 )
摘要:采用真核生物通用引物ITS1/ITS4,对来自江西省吉安县2 个车前草穗枯病菌菌株进行rDNA-ITS区段的PCR
扩增,对扩增产物进行测序,结果获得2 个长度均为579 nt且序列完全一致的rDNA-ITS序列,该序列与GenBank
中当归间座壳菌(Diaporthe angelicae) 及其无性态拟茎点霉菌(Phomopsis subordinaria)对应序列的同源性高达
99.0%,根据以rDNA-ITS序列同源性大小划分种类的标准,将车前草穗枯病菌鉴定为当归间座壳菌。
关键词:车前草穗枯病;当归间座壳菌;分子鉴定
中图分类号:S432.1 文献标志码:A 文章编号:2095 -3704 (2012 )03 -0258 -03
Molecular Identification of Pathogen of Plantain Head Blight
1 2 1 1*
LIANG Yan , TU Huai-mei , CUI Chao-yu , JIANG Jun-xi
(1. College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China;
2. Agricultural Bureau of An’yi County, An’yi 330500, China)
Abstract: In the paper, two isolates of pathogen of plantain head blight from Ji’an county in Jiangxi province
were molecularly identified. RDNA-ITS region of each of the two isolates was amplified by PCR, and the PCR
products were sequenced. Both of them are 579 nt in length, and they have a consensus sequence. The sequence
shares 99.0% homology with sequence of either Diaporthe angelicae or its anamorph Phomopsis subordinaria .
Based on the criterion of rDNA-ITS sequence homology in fungus species classification, pathogen of plantain
head blight was identified as Diaporthe angelicae .
Key words: plantain head blight; Diaporthe angelicae; molecular identification
车前草穗枯病(plantain head b
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