茶油对兔血脂及动脉粥样硬化的影响_尹岭.pdfVIP

※营养卫生 食品科学 2011, Vol. 32, No. 07 289 茶油对兔血脂及动脉粥样硬化的影响 尹 岭，张笑明，李 莉，赵秀梅 (解放军总医院医学信息情报研究所，北京 100853) 摘 要：目的：研究茶油对血脂水平和动脉粥样硬化(A S)形成的影响。方法：32 只新西兰大白兔，随机分为4 组：对照组(饲喂普通饲料) 和3 个实验油组(分别饲喂添加茶油、花生油或橄榄油的高脂饲料) ，各饲喂两个月。在 实验前、实验1 个月和实验2 个月时检测血脂。实验结束时，处死动物，取主动脉、心脏和肝脏标本，做病理 组织切片，观察组织形态学的变化，用图像分析软件测量血管粥样变化的程度。结果：喂高脂饲料的3 组大白兔 的血脂水平均明显高于普通饲料对照组(P ＜0.0 1) 。茶油组甘油三酯(TG) 、胆固醇(TC) 、低密度脂蛋白胆固醇(LDL-C) 均较花生油组低，但统计学均无显著性差异(P ＞0 .05) 。喂高脂饲料实验组的大白兔主动脉、冠状动脉都有不同程 度的动脉硬化。花生油组主动脉血管内膜增生最为明显，形成的动脉粥样斑块最大，主动脉狭窄最严重处占到血 管的3 5% ～7 0% ，而茶油组和橄榄油组主动脉血管内膜增生较花生油组低，动脉硬化程度轻，主动脉狭窄最严重 处只占整个血管的5% ～15% 。花生油组的肝细胞空泡样改变明显，呈中度脂肪肝病理改变；而茶油组和橄榄油组 肝细胞空泡样改变很轻。结论：茶油对高脂饲料诱发的肝脂肪变性和动脉粥样硬化的形成有抑制作用，其作用效 果与橄榄油相比无显著差异。 关键词：茶油；脂肪肝；动脉粥样硬化；血脂 Effect of Tea-seed Oil on Serum Lipid Level and Atherosclerosis Formation in Rabbits YIN Ling ，ZHAN G Xiao-ming ，LI Li ，ZHA O Xiu-mei (Institute of Medical Information, Chinese PLA General Hospital, Beij ing 100853, China) Abstract ：Obj ective: To investigate the effect of tea-seed oil on serum lipid metabolism and atherosclerosis (AS) formation. Methods: Totally 32 N ew Zealand rabbits were randomly divided into four group s. The rabbits in the control group were fed with normal diet and those in other three groups were fed with three kinds of high cholesterol diets containing tea-seed oil, peanut oil and olive oil, respectively, for 2 consecutive months. The serum lipid in each group was measured at 0, 1 and 2 months. At the end of the experiment, all animals were sacrificed and the aorta, heart and liver were harvested for histological examination. The morphological change was observed and the atherosclerosis status was measured by using Image-pro