胶原相关整合素α1、α2和β1在形觉剥夺性近视诱导及恢复期中调节巩膜重塑作用的研究.pdfVIP

去遮盖7天内为眼球生物学参数恢复的主要时期；豚鼠实验性近视发生时巩膜变 薄，超微结构发生变化，小直径纤维增多，表明实验性近视眼的形成是巩膜异常 重塑的过程；豚鼠后极部巩膜表达整合素 α1、α2 和 β1，豚鼠形觉剥夺诱导 期后极部巩膜整合素 α1、α2 和 β1 表达减少，去遮盖后表达上调，提示整合 素 α1、α2 和 β1 可能参与了形觉剥夺性近视的发生，在近视眼发生机制中起 了保护作用，其影响巩膜重塑的机制有待进一步研究。 硕士研究生 王园（眼科学） 指导教师 杨先 副教授 关键词：形觉剥夺性近视；整合素；巩膜；豚鼠；恢复期 万方数据 The role of collagen-binding integrin receptors in scleral remodelling during induced myopia Abstract Objective To observe the expression of the collagen-binding integrin α1、α2 and β1 in sclera of the guinea pig form-deprivation myopia(FDM), and to investigate the role of those integrins in sclera remodeling process during FDM development and recovery. Methods 160 young guinea pigs, aged 2 to 3 weeks, were randomized into experimental group (n=80) and normal control group (n=80). FDM models were monocular deprived by placement of a facemask over the right eye in the experimental group, and the fellow eyes served as the contralateral control eyes. The guinea pigs in the normal control group were raised with both eyes untreated. The FDM eyes were monocular deprived for 1, 2 and 4 weeks, and recovery 1, 3, 5, 7, 14 days after occluded 4 weeks. Optical measurements and axial length were accomplished at all of the timepoints. The histological analysis was applied to assess the posterior sclera changes, and the electron microscope was used to study the ultra-structure of sclera. The immunohistochemical staining and quan