小麦HMW-GS毛细管电泳标准图谱的构建.pdfVIP

司5 比.Ç. ~f且 2014 ， 23 (1) :80-85 Acta Agγicultuγαe Boγeali-occidentalis Sinica doi: 10.7606/j. issn.1004-1389.20 14.01.015 网络出版时间:2014-01-21 网络出版地址:/kcms/doi/ 10. 7606/j. issn. 1004-1389.2014.0 1. 015. html 小卖 HMW-GS 毛细管电泳标准图谱的构建 李艳阳，李晓燕，董 剑，陈其歧，赵万春，高 翔， 李学军，孟 敏，石引刚，陈良国 (两北农林科技大学农学院，陕西杨凌 712100) 摘 要 用毛细管电泳技术对普通小麦品种 Glu-1 位点高分子量谷蛋白亚基变异进行分析，探讨该技术对高 分子量谷蛋白亚基分离的分辨率和重复性，并构建 15 个不同常见亚基的标准图谱，试验结果增加 1Dx3 、 1Dx4 、 1Bx13 、 1Bx14 、 1By15 、 1By16 六个亚基的标准出峰时间并进行排序。对毛细管电泳图谱与 SDS 进行比较。结果表明，毛细管电泳的出峰顺序与 SDS-- PAGE 略有不同，使用该技术能简便迅速鉴定小麦高分 子量谷蛋白优质亚基，比 SDS鉴定结果更为快捷灵敏，能够高效快速测定大量不同品种的高分子量谷 蛋白亚基。 关键词 小麦;高分子量谷蛋白亚基;毛细管电泳;标准图谱 申图分类号 S512. 1+ 1 文献标志码 A 文章编号 1004-1389(2014)01-0080-06 Standard Profile of Capillary Electrophoresis for the High Molecular Weight Glutenin Subunits in Common Wheat LI Yanyang , LI Xiaoyan , DONG Jian , CHEN Qijiao , ZHAO Wanchun , GAO Xiang , LI Xuejun , MENG Min , SHI Yingang and CHEN Liangguo (College of Agronomy. \l orthwest A.F University. Yangling 5haanxi 712100. China) Abstract To provide technical support for identifying the high quality wheat glutenin subunits , capil- lary electrophoresis CCE) was used to identify some high molecular weight glutenin subunits. 1t could be used to reveal biochemical variation among Glu-1 HMW-glutenin subunits in wheat cultivars. The resolution and repeatability of capillary electrophoresis technology is analyzed and a standard profile of 15 subunits has been constructed. Based on the results , the peak time and peak order for 6 additional subunits 1Dx3 , 1Dx4 , 1Bx13 , 1Bx14 , 1By15 and 1By16 were obtained. Compared with sodium dode- cyl sulfate polyacryla