中药天年饮对衰老大鼠睾丸生精细胞端粒酶表达的影响论文.docVIP

　　中药天年饮对衰老大鼠睾丸生精细胞端粒酶表达的影响论文 庞晓静， 杨振军， 郭森， 高福禄 【关键词】 天年饮；,,衰老；,,睾丸；,,端粒酶 摘要：目的观察中药天年饮对衰老大鼠睾丸生精细胞端粒酶表达的影响。方法40只SD大鼠随机分为正常组、衰老模型组、天年饮用药组和阴性对照组，每组均为10只。采用D半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。ELISA方法检测各组大鼠血清睾酮含量、黄嘌呤氧化酶法检测血清超氧化物歧化酶（SOD）的活性、SP免疫组化法检测睾丸生精细胞端粒酶的表达。结果衰老大鼠血清睾酮含量、SOD活性明显下降（与正常大鼠相比P 0.05，P 0.01）；衰老大鼠睾丸生精细胞端粒酶的表达明显少于正常大鼠。天年饮可明显抑制衰老大鼠血清睾酮含量、SOD活性的下降（用药组与模型组相比P 0.05，P 0.01）；并能明显提高衰老大鼠睾丸生精细胞端粒酶的表达。结论 中药天年饮可明显改善衰老大鼠睾丸生精细胞端粒酶的表达，具有一定延缓衰老的作用。 关键词：天年饮； 衰老； 睾丸； 端粒酶 Effects of Traditional Chinese Medicine Tiannianyin on Telomerase Expression of Spermatogenic Cells in Testis of Aging Rats Abstract：ObjectiveTo observe the effects of traditional Chinese medicine Tiannianyin on telomerase expression of spermatogenic cells in testis of aging rats. Methods40 SD rats : normal group, aging model group, Tiannianyin group, negative control group, and each group had 10 rats. The subacutely aging model rats ade by injecting Dgal into abdominal cavity continously. ELISA , xanthine oxidase method to detect the superoxide dismutase (SOD) activity in serum, SP immunohistochemistry to observe the telomerase expression of spermatogenic cells in testis. ResultsThe testosterone content and SOD activity in serum of aging rats decreased significantly pared al rats (P 0.05,.freelerase positive cells of aging rats uch less than normal rats. Tiannianyin could inhibit the decreasing of testosterone content and SOD activity in serum significantly (Tiannianyin group pared odel group: P 0.05, P 0.01), and increase the telomerase expression significantly. ConclusionTraditional Chinese medicine Tiannianyin can improve the telomerase expression of spermatogenic cells in testis, and has fairly antiaging effects. Key erase 随着年龄的增长.freelg/kg，1次/d, 共40 d) 制作亚急性衰老的大鼠模型。模型组大鼠于造模成功后不再作任何处理；用药组大鼠于造模成功后每天用天年饮灌胃,共30 d；阴性对照组大鼠在造模成功后每天灌胃同等剂量的生理盐水,时间同用药组大鼠。 1.2 药品及试剂天年饮由何首乌、怀牛膝、肉苁蓉、淫羊藿、丹参、茯苓6味中药组成, 经熬制后每毫升含生药1.5 g按8.1 g/kg灌胃。D半乳糖，北京化学试剂公司生产（批号：020402），用生理盐水配成5%的溶液。检测血清睾酮含量的ELISA试剂盒、端粒酶免疫组化试剂盒购自北京中山生物技术有限公司。检测血清SOD活性的试