第4章_纳米物载体20100504.pptVIP

4.3.4 纳米载体的修饰与改性;4.3.4 纳米载体的修饰与改性;4.3.4 纳米载体的修饰与改性;4. 4 纳米药物载体的应用;Examples of nanomaterials and nanocarrier systems;Polymer-P/P Drug Complexes;;Lower critical solution temperature (LCST) polymers, such as poly (N-isopropylacrylamide) (PNIPAAm) with a cloud point around 32?C or some other poly (N-alkylacrylamide) compounds, were investigated as components of temperature-responsive copolymer micelles. The micelles exhibited rapid and temperature responsive drug release in cancer cells, which was caused by the destruction of the hydrophobic-hydrophilic balance with the increase of temperature. Licciardi et al. synthesized novel folic acid- (FA-) functionalized diblock copolymer micelles for target delivery of antitumor drugs. The micelles could be directly targeted delivery via folic acid and rapidly release of drug could be triggered by lowering the solution pH to 5. This strategy combined the targeted delivery of therapeutics and pH-controlled drug release together, providing a tumor-selective nanocarrier for the efficient delivery of anticancer drugs.;Schematic of drug release from micelles with temperature or pH trigger when they are uptaken by tumor cells.;Ludmila O. Cinteza et al. Diacyllipid Micelle-Based Nanocarrier for Magnetically Guided Delivery of Drugs in Photodynamic Therapy, Mol. Pharm. , 2006, 3 (4), pp 415–423;Targeted Paclitaxel by Conjugation to Iron Oxide and Gold Nanoparticles ;Cyclodextrin conjugated magnetic colloidal nanoparticles as a nanocarrier for targeted anticancer drug delivery ;Light-Regulated Activation of Cellular Signaling by Gold Nanoparticles That Capture and Release Amines ;Dendritic Polymers;纳米载体在基因治疗中的应用;adenine (A) 腺嘌呤 thymine (T) 胸腺嘧啶 guanine (G) 鸟嘌呤 cytosine (C) 胞嘧啶;1: 基因与载体结合; 2: 载体复合物被吸收到细胞内包涵体中; 3: 复合物从包涵体释放到细胞质中; 4: 细胞核吸收复合物; 5: DNA 与载体分离; 6: mRNA 表达; 7: 蛋白质表达。;纳米载体在基因治疗中的应用;基因治疗是对有缺陷的基因进行原位修复或以正常基因替代缺陷基因位点。 载体问题，以及与载体相关的免疫反应、细胞毒性与安全性等问题是基因治疗领域有待解决的关键问题之一。; ????????????????????????????????????????????????????????????????