体外反搏对高胆固醇血症猪血管内皮功能的影响论文.docVIP

　　体外反搏对高胆固醇血症猪血管内皮功能的影响论文 刘东红，伍贵富，熊艳, 罗景云, 谢强, 吕明德, 董吁钢，马虹，郑振声 【摘要】 【目的】 探讨体外反搏治疗对高胆固醇血症猪血管内皮功能的影响。【方法】 34头猪分成正常饲养组（NF, n=8）,高脂喂养组（HF, n=13）和高脂喂养+反搏组（HF+EECP, n=13）。HF+EECP组隔天反搏治疗2 h，总共反搏36 h，36 d。分别于体外反搏前（0 d）、反搏中（18 d）、反搏后（36 d），采用高频超声检测猪肱动脉血流介导的内皮依赖血管舒张功能（FMD）方法.freelg/L vs (0.469 ± 0.168) mg/L (18 d); (0.31± 0.09) mg/L vs (0.51 ± 0.26) mg/L (36 d), P 0.01。反搏后，HF组冠脉内皮细胞排列不规则、大量脱落、斑块形成, HF+EECP组内皮细胞呈流线型排列、脱落程度显著减轻。【结论】 增强型体外反搏可改善高胆固醇血症猪血管内皮功能。 【关键词】 增强型体外反搏； 高胆固醇血症； 内皮细胞； 超敏C反应蛋白； 血流介导的血管舒张功能 Abstract: 【Objective】 Enhanced External Counterpulsation (EECP) could promote shear stress to vascular endothelial cells in vivo. Hoia remains unknoent ined in porcine hypercholesterolemic model. 【Methods】Thirty-four male piglets ly divided into 3 groups: the normal feeding group (NF, n = 8), the hypercholesterolemic control group (high-fat choic + EECP” group (HF + EECP, n = 13). D) of the right brachial artery mediately after EECP treatment. High sensitivity C-reactive protein (hsCRP) easured at the same time points in all animals. At the end of study, the left descending arteries (LDA) of all animals icroscopy (SEM). 【Results】 In HF + EECP group, FMD proved significantly from 5.2％±1.7％(d0),12％±6％(d18), to 11.4％±2.8％(d36，after EECP treatment，P 0.01). On the contrary, FMD 7.8％±3.7%(d0), 7％±4％(d18), to 5.1％±2.0％(d36) in the HF group. Level of hsCRP als of HF + EECP group at day 18 and 36, paring to the HF group (0.327 ± 0.076) mg/L vs (0.469 ± 0.168) mg/L (d 18); (0.31 ± 0.09) mg/L vs (0.51± 0.26) mg/L (d 36 ), P 0.01. The distribution of LAD endothelial cells in HF piglets shoent ation of coronary endothelial cells and formation of atherosclerotic plaque after 36-day treatment. Holiner arrangement and less desquamation.【Conclusions】 EECP could improve endothelial dysfunction in hypercholesterolemic piglet model, ay benefit in preventing the development of artherosclerosis. Key ia; endothelial function; high-sensitivity C-reactive protein; floediated vasodilation 细胞内皮功能