固相酶法分离大豆胰蛋白酶抑制剂研究.pdfVIP

第29卷第6期 大豆科学 V01．29No．6 2010芷 12月 SOYBEANSCIENCE Dec． 2010 固相酶法分离大豆胰蛋白酶抑制剂研究 陈 滴，刘银燕，杨锦竹，王广树，杨晓虹 (吉林大学药学院，吉林长春130021) 摘要：以硅胶一壳聚糖为载体，通过戊二醛偶联胰蛋白酶，制备固定化酶，分离纯化大豆中胰蛋白酶抑制剂。对戊 二醛浓度、反应温度、酶浓度等影响固定化酶制备的因素进行了研究。戊二醛的浓度为2．O％，反应温度为20％， U·mg～，比粗品高46．22 胰蛋白酶与载体比为15：l000时，分离纯化的大豆胰蛋白酶抑制剂活性最高，达1872．51 倍。结果表明通过该法能够获得较高活性大豆胰蛋白酶抑制剂，为大豆胰蛋白酶抑制剂的开发利用提供了依据。 关键词：固相酶法；大豆；胰蛋白酶抑制剂 中图分类号：TQ646．5 文献标识码：A InhibitorswithSolid-PhaseMethod SeparatingSoybeanTrypsin Enzyme CHEN Di，LIU Jin—zhu，WANG Yin—yan，YANG Guang-shu，YANGXiao-hong ofPharmacuetical (School Sciences。JilinUniversity，Changchun130021，Jilin，China) orderto and the was sili· Abstract：In soybean inhibitors，immobilizedtrypsinpreparedby separatepurify trypsin coupling conechitosanactivatedcarrierswith as influencefactorsof reaction trypsinusingglutaraldehydecouplingagents．The coupling and and concentrationwere theresultsshowed concentration，reactiontemperaturetrypsin investigated includingglutaraldehyde conditionswereas concentrationof was was theratio thatthebest Allow：the 2．0％。the 20％，and glutaraldehyde temperature the of withsilicone-chitosanwas15：1000．The of inhibitorsisolated aboveimmobilized trypsin activitysoybeantrypsin by 1872．51 the increased46．22times．Theresults that in· was