陆地棉耐盐相关基因GhERF79的克隆与分析.pdfVIP

花 学 报 Cotton Science 20 13 25 渊2 冤院95~ 102 地棉耐盐相关基因 79 的克隆与分析 洁琳 邹长松 陆才瑞 巧莲 宋国立* 国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁河南安阳455000 冤 摘要院利用RACE-PCR 技术克隆陆地棉ERF 基因的全长cDNA 袁命名为 79 袁该cDNA 长度为980 bp 袁 含有一个完整的ORF Open reading frame 冤为756 bp 袁编码251 个氨基酸的多肽曰亚细胞定位显示该基因表达 产物定位于细胞核 袁生物信息学分析表明 79 属于ERF 转录因子家族内的A-1 亚族遥 Real-time PCR 分析结果表明袁 79 的表达受盐胁迫和植物激素诱导袁 该基因在耐盐材料 9806 的诱导表达水平显 著 于盐敏感材料中棉所12曰外源激素ABA Abscisic acid 冤尧ET Ethylene 冤尧MeJA Methyl j asmonate 冤可以显著 诱导 79 的表达袁推测其可能在棉花响应盐胁迫途径 发挥重要功能遥 关键词院陆地棉曰盐胁迫曰 79 曰ERF 曰克隆 中图分类号院S562.035.3 文献标志码院A 文章编号院1002-7807(2013)02-0095-08 WANG Jie-lin, ZOU Chang-song, LU Cai-rui, WANG Qiao-lian, SONG Guo-li* 渊 455000, 冤 In this study, the full-length cDNA sequence of 79 was isolated from by RACE-PCR, its length is 980 bp, and it has an open reading frame (ORF) of 756 bp encoding a protein of 251 amino acids which was located in nucleus by subcellular localization experiment; Bioinformation analysis revealed that 79 belonged to A-1 subgroup of ERF subfamily; Expression pattern analysis revealed that the expression of 79 was significantly induced by salt stress and plant hormones ABA, ET, and MeJA, furthermore, the induced expression level in salt-tolerance germplasm Zhong 9806 ( ) was considerably higher than that in salt-sensitive cultivar CCRI 12 ( ). The evidence above suggested that 79 might play an important role in regulating cotton salt stress responses. ; salt-stress; 79; ERF; clone 植物在整个生长发育过程中会受到各种逆 乙烯应答曰该产物被命名为EREBP[2] 渊Ethylene re- 境胁迫的影响 导致其产量