甜菜单体附加系M14种子形成方式.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2013, 39(3): 381388 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.00381 甜菜单体附加系M14 种子形成方式 马 兰1,2,** 杜洪岩1,** 李荣田1,* 1 / , 150080; 2 , 163000 : M14 , F1 ; SSR M14 , M14 ; , F1 , M14 ; M14 SSR M14 Southern , M14 DNA, M14 M14 9 : M14; ; ; ; ; Southern Seed-Setting Mode of Monosomic Addition Line M14 of Beta corolliflora in Sugar Beet MA Lan1,2,**, DU Hong-Yan1,**, and LI Rong-Tian1,* 1 Key Laboratory of Molecular Biology, College of Heilongjang Province / Heilongjiang University, Harbin 150080, China; 2 Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163000, China Abstract: A cross combination of M14 with green petiole ×B. vulgaris with red petiole was prepared to observe the dominant trait marker of hybrid plants, and the genetic correlation between the maternal plant and F generation of M14 based on the SSR analy- 1 sis. The result showed that M14 had higher transmission rate of the alien chromosome. Multiplied seeds came from the union of male and female gametes under artificial pollination, and the petiole color of all the hybrid plants was red. There was no the ex- actly identical genotype in the progeny compared with the maternal plant. It indicated that the seed-setting mode of M14 is not apomixis, but sexual reproduction. Southern blot showed that the chromosomes of B. vulgaris reversed from M14 sharing the DNA sequence of B. corolliflora, it is