小麦Mlo反义基因的转化及转基因植株的白粉病抗性分析.pdfVIP

作物学报 ACTA AGRONOMICA SINICA 2013, 39(3): 431439 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2013.00431 小麦Mlo 反义基因的转化及转基因植株的白粉病抗性分析 邢莉萍 钱 晨 李明浩 曹爱忠 王秀娥 陈佩度* , 210095 : Mlo 158 20 , , PCR PCR-Southern DNA BASTA 158 20 , Mlo T0 T1 , 6 T1 , Mlo , , Mlo : ; Mlo ; ; Transformation of Antisense Wheat Mlo (Ta-Mlo) Gene and Wheat Powdery Mildew Resistance Analysis of Transgenic Plants * XING Li-Ping, QIAN Chen, LI Ming-Hao, CAO Ai-Zhong, WANG Xiu-E, and CHEN Pei-Du National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China Abstract: The antisense wheat Mlo gene, Ta-Mlo, was transformed into wheat (Tritivum aestivum L.) varieties Yangmai 158 and Jimai 20 via biolistic transformation using immature embryo calli as explants. After two rounds of bialaphos selection and regen- eration, herbicide-resistant plants were obtained, which were subsequently confirmed by PCR, PCR-Southern hybridization, ge- nomic dot hybridization, and BASTA resistance analysis. The results showed that the Ta-Mlo antisense transgenic Yangmai 158 and Jimai 20 plants were obtained. The real time fluorescence quantitative PCR analysis proved that the transcript of Ta-Mlo was knocked down in these transgenic plants. The disease resistance test showed that the six transgenic lines appeared highly resis- tance to powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt). The transgenic lines showed distinct acceleration of the production and stabilization of papillae, and