左向右分流型肺动脉高压大鼠肺组织中microRNA的表达谱 .docVIP

左向右分流型肺动脉高压大鼠肺组织中microRNA的表达谱卢荣鑫，钟前进（400042重庆，第三军医大学大坪医院野战外科研究所心血管外科） 检测晚期肺动脉高压（pulmonary hypertension，PH）大鼠肺组织中microRNA （miRNA）的表达，初步预测差异表达的microRNA调控的靶基因。方法4～周龄健康雄性SPF级SD大鼠20只，体90～110g，分成分流组和对照组建立左向右分流型肺动脉高压大鼠模型12周后，取大鼠外周肺组织，运用microRNA表达谱芯片检测分流组和对照组大鼠肺组织microRNA的差异性表达。运用Miranda、TargetScan、PicTar软件预测可能调控的靶基因，实时定量PCR验证miR-98、miR-130b和miR-127等的表达。结果平均肺动脉压（mPAP）、肺动脉中膜厚度百分比（MT%）及右室肥厚指数（RVHI）均明显高于对照组（P 0.01），microRNA芯片结果提示：与对照组相比，在分流组大鼠肺组织中表达明显上调的miRNA有3个miR-122、miR-130b、miR-146b，明显下的miRNA有7个miR-382、miR-192、miR-29c，RT-PCR结果与芯片结果一致。结论 microRNA在左向右分流型肺动脉高压大鼠中表达存在差异，microRNA可能参与肺动脉高压大鼠肺血管的重构。关键词肺动脉高压microRNA；肺血管重构 microRNA Expression Profiles in peripheral pulmonary tissues of rats with pulmonary hypertension induced by left to right shunt [Abstract] Objective To investigate the differential expressions of micrRNAs in peripheral pulmonary tissues of rats with pulmonary hypertension (PH) induced by left to right shunt. Methods Twenty male SD rats (aged 4 - 5 weeks，weighing 95-110 g) were randomly divided into shunt group and control group (10 each). Rats in shunt group underwent right common carotid artery-external jugular vein connection to reproduce PH model of left to right shunt, while animals in control group only received sham operation. The peripheral pulmonary tissues were collected 12 weeks after the operation. Quantitative real-time PCR were performed to confirm the results obtained by microarray analysis. Meanwhile, their target genes were predicted by miRNA target gene prediction softwares such as MIRanda, TargetScan and PicTar. Results Mean pulmonary arterial pressure (mPAP), right ventricular hypertrophy index (RVHI), percentage of small pulmonary arteries media thickness (MT%) were significantly increased in shunt group compared with control group after 12 weeks shunt(P0.01). MicroRNA microassay showed that 37 microRNAs were differentially expressed in shunt group and control group including a set of 30 overexpressed microRNAs and 7 downexpressed miRNAs. RT-