酵母菌Y17吸附Cu2+的影响因素和吸附机理研究.PDFVIP

微生物学通报 MAY 20, 2008, 35(5): 772~776 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 酵母菌Y17 吸附Cu2+ 的影响因素及吸附机理研究 * 肖 宁 陈 强 裴浩言 蒋 玮 ( 625014) 摘 要: 以高效吸附Cu2+ 的酵母菌Y17 为材料, 对其吸附 Cu2+ 过程中的主要影响因素, 包括溶液 pH 、Cu2+初始浓度、菌体添加量、吸附时间和温度以及吸附机理进行了探讨。结果表明, 对吸附 过程影响较大的因素依次为吸附液 pH 值、Cu2+初始浓度、菌体添加量和吸附时间。正交试验得 到最佳吸附条件为溶液pH5.0, 吸附时间40 min, 加菌量5.0 g 湿菌/L 时, 对初始浓度为 8 mmol/L 的Cu2+ 达到最佳吸附率为82.7%。通过对Y17 菌体不同处理及解吸实验, 初步确定Y17 吸附Cu2+ 的位点在细胞壁, 细胞壁表面的−NH , −COOH 基团在其吸附过程中起着重要作用。 2 关键词: 酵母菌, 微生物吸附, 铜离子, 正交试验 Influence Factors and Absorbing Mechanics of Copper Ion by Yeast Strain Y17 * XIAO Ning CHEN Qiang PEI Hao-Yan JIANG Wei (College of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan 625014) Abstract: Using the high efficient copper-adsorbing yeast strain Y17 as absorbing material, the major affect factors including pH, original concentration of Cu2+, cell biomass, adsorption time and temperature were examined, and then the absorbing sites of the Y17 was determined. The results showed that the solution pH was the most dominate factor which affected the biosorption of Cu2+, the other affecting factors were the ini- tial concentration of Cu2+, the cell biomass added, and adsorption time, respectively; the temperature had lit- tle effect on the rate of bi