反相高效液相色谱法测定溪黄草中二萜类成分的含量论文.docVIP

　　反相高效液相色谱法测定溪黄草中二萜类成分的含量论文 林朝展 祝晨蔯 杨金燕 【摘要】 目的通过溪黄草中二萜成分含量测定方法的建立及商品药材质量分析，为溪黄草质量标准的制定提供依据。方法采用RPHPLC法测定6,7去氢罗伊林酮和(15S)12,16环氧11,14二羟基8,11,13松香烷7酮的含量；流动相：0.05%三氟乙酸乙腈（25∶75），检测波长：210 nm；理论塔板数按6,7去氢罗伊林酮计算应不低于6 000，按(15S)12,16环氧11,14二羟基8,11,13松香烷7酮计算应不低于5 000。结果11批次的测试样品中6,7去氢罗伊林酮的含量范围在91.5～182.5 μg·g-1，(15S)-12,16环氧11,.freelethod for determination of diterpenoids in different batches of the aerial part of Isodon lophanthoides var. gerardianus. MethodsAn RPHPLC method ination of 6,7Dehydroroyleanone and (15S)12,16Epoxy11,14dihydroxy8,11,13abietatrien7one by using Kromasil RPC18(250 mm×4.6mm.freel) column, ixture of 0.05% trifluoroacetic acidAcetonitrile (25∶75) as mobile phase at the flol·min-1 and detection .ResultsThe contents of the above tong the 11 patches of tested samples. The content of 6,7Dehydroroyleanone ranged from 91.5 to 182.5 μg·g-1 and that of (15S)12,16Epoxy11,14dihydroxy8,11,13abietatrien7one ranged from 35.4 to 84.2 μg·g-1. The suggested content limitation of 6,7-Dehydroroyleanone should be 113.4 μg·g-1, and that of (15S)12,16Epoxy-11,14dihydroxy8,11,13abietatrien7one be 52.3 μg·g-1 in the crude Isodon lophanthoides var.gerardianus.ConclusionThe established method has been proved to be simple, stable and repeatable, and can be applied for quality control of the crude Isodon lophanthoides var.gerardianus and pound prescriptions contained crude Isodon lophanthoides var.gerardianus. Key ination； RPHPLC 溪黄草为唇形科香茶菜属植物狭基线纹香茶菜 Isodon serra（Maxim）Hara的干燥全草，具有清热利湿、退黄、凉血散淤之功效，是我国南方地区治疗乙型肝炎的常用草药［1］，临床应用广泛，疗效确切，目前已有较多的以其为主要原料的养肝护肝中药制剂及保健品。该植物主要含有松香烷型二萜类（Abietane type）及乌索烷型三萜类成分(Ursane type) ［2］，迄今为止国内外学者报道已从中分离得到13种二萜和4种乌索烷型三萜，其中6个松香烷型二萜（Abietane type）分别为狭基线纹香茶菜甲素、乙素（Lophanthoidin A、B），11βhydroxyl8，15isopimaradiene 3one、Gerardianin A，Gerardianin B，Lophanic acid，1个半日花烷型二萜（Labdane type）即8(17),12,14Labdatriene19oic acid及6个二萜醌分别为16Acetoxy7O acetyl horminone，16Acetoxy12Oacetylhorminone，Roylean