山羊肺泡巨噬细胞体外分离与原代培养技术研究重点讲义.doc

安徽大学 本科毕业论文（设计、创作） 题　　目：山羊肺泡巨噬细胞体外分离与原代培养技术研究　　　　　　　　 　　　　　 　　　　 学生姓名：　　孙义　　 学号： 院（系）： 　生命科学学院　 专业：　生物工程 　　　　 入学时间：　 2012　　　　　年　　　9　　　　月 导师姓名：　　班谦　　 　　 职称/学位：　 博士　 　 导师所在单位：　　 　安徽大学　　　　 　　　　　　 完成时间：　 2016　　　　年　　　5　　　　　月 山羊肺泡巨噬细胞体外分离与原代培养技术研究 摘要 肺泡巨噬细胞是存在于肺泡中的免疫细胞，它是免疫系统的重要组成部分，肺泡巨噬细胞免疫功能非常活跃。它是肺脏抵御外来微生物侵害的第一道防线。本次实验通过研究山羊巨噬细胞的体外分离和体外培养技术，从而建立一套完整可行的巨噬细胞分离培养技术方法。 人体吸入空气中的尘粒、细菌等异物进入肺泡和肺间质，多被巨噬细胞吞噬清除Isolation and primary culture of goat alveolar macrophages in vitro Abstract Alveolar macrophages are present in alveolar in immune cells, it is an important part of the immune system, immune function of alveolar macrophages is very active. It is the first line of defense against microbial invasion of the lung. This experiment through study on goat macrophages in vitro isolation and in vitro culture techniques, so as to establish a complete set of feasible macrophage separation cultivation techniques. Inhaled dust particles in the air, bacteria and other foreign matter into the alveolar and interstitial lung, is phagocytosis of macrophages, in todays air pollution increasingly serious situation, study of alveolar macrophages is important for immunology and human health. And the study of macrophage isolation technology will lay a solid foundation for Immunology Study on alveolar macrophages. The separation of alveolar macrophages from healthy lamb lung lavage fluid of lesions in this experiment, With lipopolysaccharide induced stimulation. The experimental results show that through the experimental method, goat alveolar macrophages in high purity and in CO2 culture box in continuous culture about 30 days found no large-scale cell death, under the stimulation of LPS found it still has the immune activity. Study on the immunological function of further by goat alveolar macrophages were all pathogenic bacteria caused the disease treatment and alveolar macrophages lay the experimental foundation.? Keywords: alveolar macrophages ; isolated cul