二十世纪七十年代以前出版的中文版中药书籍中的药用青蒿只有一种.doc

黄花蒿与其近缘种化学成分的FTIR和 GC-MS鉴定与分析 孔德鑫1，李雁群2，邹蓉1，史艳财1，韦霄1* （1.广西植物功能物质研究与利用重点实验室，广西植物研究所，桂林，514006; 2. 亚热带农业生物资源保护与利用国家重点实验室，华南农业大学，广州，510642(） 摘要：黄花蒿是一种治疗痢疾的特效中药植物体中含有丰富的精油但其应用和生产中常有种类混杂现象，严重影响了黄花蒿为原料的药材质量。为实现黄花蒿药材快速鉴定与评价。本研究利用FTIR技术和GC-MS分别对不同种黄花蒿及近缘种叶片原药材及挥发油成分进行检测和鉴定。挥发油以黄花蒿含量最高（1.86%），其次是南牡蒿、茵陈蒿、青蒿、牡蒿和艾蒿。FTIR分析结果表明，黄花蒿及近缘种一维图谱相似，酰胺类、芳香类以及萜类化合物种类较多且含量丰富；二阶导数图谱中，黄花蒿青蒿素成分振动吸收明显增强，可以明显将黄花蒿与其混淆中区分开。GC-MS分析显示，黄花蒿与其近缘种的挥发油成分中共检测出17个共有峰，28种化学成分，均含有较高樟脑、á-杜松烯、Crocetane、植烷、2,4-二叔丁基苯酚，但不同种间成分含量差异很大，植烷在黄花蒿中含量明显高于其它近缘种，龙脑成分只能在黄花蒿叶片中检测出，然而，á-雪松烯在青蒿、南牡蒿、茵陈蒿均较高，而在黄花蒿，艾蒿，牡蒿中含量均较低。最后通过聚类分析探讨了黄花蒿与其近缘种挥发油成分差异性，6 种材料明显聚为2类，其中，黄花蒿与牡蒿、艾蒿聚为一类，青蒿与茵陈蒿和南牡蒿聚为一类。本研究结果为黄花蒿药材的真伪鉴别及其药材质量评价提供了快速而有效的分析手段。 关键词：黄花蒿挥发油GC-MS，红外图谱质量评价 GC–MS and FTIR identification and analysis of chemical component in Artemisia annua L. and its closely related species KONG De-Xin1, LI Yan-Qun2, ZOU Rong1, SHIYan-Cai1, WEI Xiao1* 1.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Chinese Academy of Sciences, Guilin,541006；2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China） Abstract: Artemisia annua L. contain abundant essential oils. It is commonly used as traditional Chinese medicine for treating dysentery. The varietal complexity and vague provenance boundaries of the species of A. annua seriously affected the quality of medicinal materials. To investigate the rapid identification and evaluation of A. annua and sibling species leaves on the yields and compositions of essential oils, the volatile compounds were detected and identified by GC–MS and FTIR analysis. A. annua determined to have the highest yield (1.86%) of essential oil, followed by A. eriopoda, A. capillarie, A. apiacea, A. japonica and A. argyi. FTIR results showed that one-dimensional spectrum was similarity of A. annua and sibling species and the species were abundant of amides, aromatics and terpen