芯片技术与肿瘤中DNA甲基化研究.PDFVIP

HEREDITAS (Beijing) 2008 3 , 30(3): 295―303 ISSN 0253-9772 综述 DOI: 10.3724/SP.J.1005.2008.00295 DNA 田筱青, 孙丹凤, 张燕捷, 房静远 , , 200001 : DNA 甲基化是表观遗传学的一个重要部分。它参与基因转录调控, X 染色体失活, 发育调控及细胞分化 的过程。异常的DNA 甲基化与癌症的发生密切相关。芯片技术的发展为高通量研究DNA 甲基化提供了新的 方法。各种芯片技术以不同的 DNA 预处理方法为基础, 包括免疫沉淀和限制性内切酶等。免疫沉淀方法特异 性高, 而限制性酶的方法具有较高的灵敏度。虽然每种方法都有一定的局限性, 但是它们为在基因组范围研究 癌症的甲基化谱提供了更多的选择。 : DNA 甲基化; 芯片; 免疫沉淀; 限制性内切酶; 癌症 Microarray-based methods to identify DNA methylation in cancer TIAN Xiao-Qing, SUN Dan-Feng, ZHANG Yan-Jie, FANG Jing-Yuan Shanghai Jiaotong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Diseases , Shanghai 200001, China Abstract: DNA methylation is an epigenetic modification associated with gene transcription regulation, X-chromosome inactivation, regulation of development and cell differentiation. Aberrant DNA methylation has been linked to cancer. The advent of microarray technology has provided new opportunities for high-throughput study on DNA methylation. Various microarray-based methods depend on different pretreatments including immunoprecipitation and restriction digestion. Al- though each technique has its limitations, the immunoprecipitation method has high specificity, and the restriction digestion method shows high sensitivity. Together they provide many choices to the study of genome-wide DNA methylation profile in cancer. Keywords: DNA methylation; microarray; immunoprecipitation; restriction enzyme; carcinoma DNA , DNA DNA 10 , DNA