罗汉果不同器官直接分化再生苗的研究.PDFVIP

生物技术通报 BIOTECHN OLOG YB ULLETIN 2006 * 蓝桃菊许鸿源 何冰林纬黎起秦沙波 (, 530005) : 以青皮果品种的叶片叶柄和无芽茎段作 外植体, 研究其直接分化再生苗能力的差异结果表 明: MS + 6-BA 1. 0 mg / L + IAA 0. 1mg / L和 M S + 6-BA 2. 0 m g / L + IAA 0. 1m g / L 两组培养基均可使 叶片外植体直接分化出芽并建成再生苗叶片基部的分化能力最强, 中部稍次, 尖部最弱 MS + 6-BA 3. 0 mg / L + IAA 0. 1m g / L则诱导叶片外植体首先脱分化形成愈伤组织, 继而再分化成再生苗, , 且畸形苗居多, 无应用 价值 叶柄和无芽茎段在三组培养基中都只能脱分化形成大量愈伤组织, 难以分化再生苗 : 罗汉果 再生苗 器内培养 Studies on D irect D ifferentiatiing R egenerated P lantlets From D ifferent O rgan s of sira itia grosvenorii in vitro Lan TaojuXu H ong uanH e Bing LinW ei L iQ iqin Sha Bo (A g ricu ltural colleg e , Guangx i Un iv ersity, N ann ing 530005) Abstract: Leaf, petiole and stem cuttings( w ithout buds) of S iraitia grosvenorii w ere used as explants, in order to stud their d ifferentiate abilities of regenerate p lantlet in vitroThe resu lts show ed that: ( 1) On m edium MS + 6-BA 1. 0 m g / L+ IAA 0. 1 mg / L and M S + 6-BA 2. 0 m g / L + IAA 0. 1mg / L, regenerated p lantlets cou ld be d irectl in- duced from the segm ents of lea.f The base part segm ents ofLeaf show ed strongest abilit in d ifferentiating regenerated p lant- lets, then w as them edium part segm ents and last w as the tip part segm en ts. ( 2) M ed ium M S + 6-BA 3. 0 m g / L+ IAA 0. 1m g / L induced firstl the segm ents of leaf to form callus, and then the callus differentiated regenerate p lantlets, but m ost of the plan tlets w ere deform it . ( 3) Petioles and stem cuttings (w ithou t buds) easil form ed a great quantit of callus on the above three m ed ia, but d ifficu lt to form regenerate plan tlets