黄瓜果实成熟衰老过程中几种物质的变化.pdfVIP

中国蔬菜 2010（12）：41-45 CHINA VEGETABLES 黄瓜果实成熟衰老过程中几种物质的变化 王志坤 1，2 秦智伟 2* 周秀艳 2 1 2 （东北农业大学大豆生物学教育部重点实验室，黑龙江哈尔滨 150030；东北农业大学园艺学院， 黑龙江哈尔滨 150030） 摘 要：以黄瓜品种 D0313 和 649 为试材，对果实自然成熟衰老过程中外观形态和若干重要生理生化 指标进行了研究。结果表明，两个黄瓜品种的脂氧合酶（LOX）活性前期上升后期下降；可溶性蛋白质含 量有个迅速下降过程；谷氨酰胺合成酶（GS）活性前期保持稳定，衰老后期急剧上升。两个品种之间变化 趋势基本一致，其中存在的差异可能是造成两个品种果实衰老表现不同的原因所在。初步认为，膜脂过氧 化作用是导致黄瓜果实衰老的主要生理原因之一。 关键词：黄瓜果实；衰老；生理生化变化；膜脂过氧化 中图分类号：S642.2 文献标识码：A 文章编号： 1000-6346（2010）12-0041-05 Changes of Several Substances during Ripening and Senescence Process of Cucumber Fruit 1, 2 2* 2 WANG Zhi-kun , QIN Zhi-wei , ZHOU Xiu-yan 1 （Key Laboratory of Soybean Biology, Northeast Agricultural University, Ministry of Education, Harbin 150030, Heilongjiang, China; 2Horticulture College of Northeast Agricultural University, Harbin 150030, Heilongjiang, China ） Abstract ：In this paper, fruit morphology and several important physiological and biochemical identification indexes correlating with ripening and senescence were monitored for cucumber（ Cucumis sativus L.）fruits from two different cucumber cultivars D0313 and 649. The results showed that LOX activities were increasing in early stage and decreasing later. The contents of soluble protein in these two varieties had a speedily decreasing process. GS activity was stabilizing in early stage and increasing rapidly in later stage of senescence. The changing trends of these two cucumber varieties were identical, but the existing differences between them may be the causes for their fruit different senescence conducts. The peroxidation of membrane lipid was considered to be one of the major physiological causes leading to senesc