狼毒大戟根提取物抑菌活性及其提取方法初探.pdfVIP

中国蔬菜 2010（16）：67-73 CHINA VEGETABLES 狼毒大戟根提取物抑菌活性及其提取方法 初探 * 孙瑞艳 陈亚彤 范海延 于佳霖 潘 健 （沈阳农业大学生物科学技术学院，辽宁沈阳 110161） 摘 要：以连续回流提取法从狼毒大戟根中提取得到抑菌活性物质，采用生长速率法进行离体抑菌试 验确定其对多种植物病原真菌的抑菌活性。狼毒大戟根提取物对黄瓜枯萎病菌、菜豆炭疽病菌、甜瓜枯萎病 菌等 9 种供试病原真菌菌丝生长均有一定的抑制作用。通过单因素试验和正交试验确定最优提取工艺为：以 90 %乙醇作为溶剂，料液比 1︰20，在提取温度为 65 ℃和提取溶剂 pH 为 10.0 条件下提取 90 min，提取次数 为 2 次。 关键词：狼毒大戟；有机溶剂提取物；抑菌活性；黄瓜枯萎病菌；菜豆炭疽病菌；甜瓜枯萎病菌 中图分类号：S482.2+92 文献标识码：A 文章编号：1000-6346（2010）16-0067-07 Initial Studies on the Antifungal Activities and Extraction Method of Extracts from Euphorbia fischeriana Root * SUN Rui-yan, CHEN Ya-tong, FAN Hai-yan , YU Jia-lin, PAN Jian （School of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110161, Liaoning, China ） Abstract ：In this paper，the bioactive substances obtained from Euphrbia fischeriana root were tested for antifungal activities against plant pathogens through growth rate law. The main factors influencing bioactive substances extraction and their best levels were confirmed by single factor analysis and the optimum condition was confirmed by orthogonal test. The research indicates that extracts of Euphrbia fischeriana root have inhibitory activities to many kinds of plant pathogen. In particular, inhibitory rate of Fusarium oxysporum f. sp. cucumerinum, Colletotrichum lindemuthianum and Fusarium oxysporum f. sp. melonis is better comparatively. The results showed that the optimal conditions were: 1∶20 of 90 % ethanol to sample weight, extraction temperature 65 ℃, pH value 10.0, extraction dura