转录组学技术及其在瓜菜作物上的应用.pdfVIP

2016 ,29(12): 1-5 , 13 专题综或 中国瓜菜 转录组学技术及其在瓜菜作物上的应用 张艳艳 12 ，季苇芹口杨玉文 2 ，关 巍 2 ，赵廷昌 2 (1.吉林农业大学农学院 长春 130118; 2，植物病虫害生物学国家重点实验室· 中国农业科学院植物保护研究所 北京 100093) 摘 要:转录组学技术作为植物功能基因组学研究的重要手段，近年来在瓜菜作物上也有不少成功应用。运用转录 组学技术，可以从转录水平上探究瓜菜作物在特定发育阶段或特定生理状态下的基因功能和基因结构，从而为了解 瓜菜作物在不同条件下的发育调控及胁迫响应机制提供数据基础。本文主要介绍了转录组学的技术方法及转录组 学技术在西瓜、甜瓜、黄瓜、番茄和西兰花等瓜菜作物上的应用，最后展望了转录组学技术在瓜菜作物研究中的应用 前景。 关键词:瓜菜作物;转录组学;应用 The technology of transcriptome and its application in cucurbit and vegetable crops 1 1 2 2 2 ZHANG Yanyan ,2 ,]1 Weiqin YANG Yuwen ,GUAN Wei ,ZHAO Tingchang 气 (1. lilinAgricultural University , Changchun130118,Jilin, China; 2. State Key Labora归ryforB切10gyofPIαntDiseαses αnd lnsect Pests ,Institute ofPlαnt Protection. Chinese Academy ofAgricultural Sciences ,Beijing 100093 , Chinα) Abstract: Transcriptome has been developed as an powerlul tool for plant functional genomics , and also successfully applied in cucurbit and vegetable crops , We can explore gene function and structure of cucurbit and vegetable crops in specific developmental stages or specific physiological state at transcriptionallevel by this technique. Accordingly ,we can understand the development regulation and the stress response mechanism of cucurbit and vegetable crops under different enviroment. This paper mainly summarise the methods of transcriptome sequencing and the application in cucurbit and vegetable crops. Finally •the prospect of the transcriptome in cucurbit and vegetable crops research is proposed. Key words: Cucurbit and vegetable crops; Transcriptome; Application 随着功能基因组时代的到来，转录组学、蛋白