TargetingInducedLocalLesionsInGenomes(TILLING)for分析.pptVIP

The Authors Steven Henikoff Basic Sciences division of the Fred Hutchinson Cancer Research Centre in Seattle Washington Currently working to expand TILLING to other organisms Believes that TILLING could improve certain crops through gene knockouts and alterations, while not needing to insert foreign DNA. This could alleviate pressure from groups lobbying against GMOs The Authors Claire M. McCallum Developed technique while a graduate student in Dr. Henikoff’s lab Was discouraged when trying to create gene knockouts of genes coding for chromomethylases (possible role in silencing) Developed TILLING to study role of chromomethylases by creating allelic series of target genes The Authors Elizabeth A. Greene Currently working in bioinformatics field at the Fred Hutchinson Cancer Research Centre in Seattle Washington Created program to calculate that a gene segment will contain a damaging mutation Honorable Mention Bradley Till Runs the TILLING project at the University of Washington on a day to day basis Provides free workshops to the research community in order to facilitate the use of TILLING for other organisms Did not invent TILLING technique Provides excellent bus directions to Key Arena for basketball games, complete with map, schedule of times, and much more Loves Canadian beer Aim of the Paper To introduce a new technique useful for creating an allelic series of gene disruption/knockout to the scientific community Raising interest in the the technique to generate new ideas for improvement of TILLING, and expand TILLING to other organisms Provide insight into possible uses for TILLING, such as genetic modification of crops TILLING Overview Mutagenesis First you need to have a mutagenized population from which to begin the process Typically, you want to have a rate of one mutation per 300,000 bp when creating your population. A good mutagenesis efficiency lowers costs, but too much mutation causes problems in progeny (lethals, poor growth, higher need for outc