柠檬酸转运载体在蟑螂保幼激素合成中研究进展.pdfVIP

Bioprocess 生物过程, 2017, 7(1), 1-7 Published Online March 2017 in Hans. /journal/bp /10.12677/bp.2017.71001 Functional Characterization of Citrate Carrier in the Biosynthesis of Juvenile Hormone in Cockroach Shicheng Zhang1,2, Juan Huang2 1 College of Life Science of Hebei University, Baoding Hebei 2 Institute of Zoology, Chinese Academy of Science, Beijing th th th Received: Mar. 8 , 2017; accepted: Mar. 26 , 2017; published: Mar. 29 , 2017 Abstract Purpose: To determine the function of Citrate carrier (CiC) in the biosynthesis of Juvenile hormone in cockroach, D. punctata, and build a basic theory for pest control. Methods: The expression pro- file and tissue distribution of CiC were determined by qRT-PCR. Furthermore, the relationship between JH biosynthesis and CiC were investigated by knocking down the essential genes in JH biosynthesis pathway using RNAi. Results: CiC has the highest expression level in CA, which is the biosynthetic site of JH, and followed by Fb. CiC expression pattern in CA corresponds with JH bio- synthesis in the CA of 0 - 7d after mated female. There is a down regulation in CiC expression after knocking down the 3-hydroxy-3-methylglutaryl coenzyme-A reductase and Juvenile hormone acid methyl transferase, two key genes that play essential roles in JH biosynthesis. Conclusion: Our re- sult suggested CiC was involved in JH biosynthesis pathway, probably by mediating the trans- membrane transport of Acetyl-CoA. Further investigate was needed to determine the accurate function of CiC. Keywords Citrate Carrier, Juvenile Hormone, Corpora allata, RNAi 柠檬酸转运载体在蟑螂保幼激素合成中的