分光光度法测定夏枯草中总黄酮的含量论文.docVIP

　　分光光度法测定夏枯草中总黄酮的含量论文 邓斌，蒋刚彪，黄红英，陈六平 【摘要】 目的研究可见光及紫外光分光光度法测定夏枯草中总黄酮含量的方法。方法可见光分光光度法是以芦丁为参照品,硝酸铝作显色剂，在510 nm波长处测定总黄酮含量；紫外光分光光度法是以芦丁为参照品，在357nm波长处测定总黄酮含量。结果可见光法平均回收率为97.76%，紫外光法为99.10%，前者的RSD值是1.02%.freeline the total flavone content in Prunella vulgaris L. by VIS and UV Spectrophotometry.MethodsThe control sample of VIS Spectrophotometry .The control sample of UV Spectrophotometry .ResultsThe average recoveries of VIS and UV Spectrophotometry etry is simple, rapid, accurate, economical and is suitable for content determination of total flavones in Prunella vulgaris L.. Key etry 中药夏枯草为唇形科夏枯草属