面向POCT应用的微流控芯片技术综述.pdfVIP

年年 33 月月 第第 11 卷卷 创刊号创刊号 30 World Journal of Complex Medicine World Journal of Complex Medicine ，，Mar.2015Mar.2015，，Vol.1Vol.1 ，，No.1No.1 * 综述* 面向POCT应用的微流控芯片技术综述 李晓琼，杨春华，潘邵武，韩杰，况玉香，施青峰 北京理工大学生命学院，北京 100081 摘要 即时检测 (POCT) 以实现快速便捷的临床现场检验为主要目标，具有检测实时性高、综合成本较低、不依赖专业设备 等优势，广泛应用于临床监护、检验检疫、家庭保健等领域。微流控芯片技术因其样品消耗量小、检测灵敏度高、易于集 成和实现自动检测等特点，已成为实现 POCT 的重要技术手段。本文根据核酸、蛋白、细胞等检测对象的属性特点，分类 综述了面向 POCT 应用的微流控芯片检测技术研究现状。进而针对 POCT 临床应用需求，介绍了 POCT 用微流控芯片在芯 片构建技术、系统化、产品化等方面的研究成果。最后，针对该领域的研究难点和发展趋势进行了讨论。 关键词 即时检测 (POCT) ；微流控芯片；核酸检测；蛋白质检测；细胞检测；产品化 中图分类号 V448.15 文献标志码 A doi 10.11966/j.issn.2095-994X.2015.01.01.05 Microfl uidic Lab-on-a-chip Technologies for Point-of-care Testing System: A Review LI Xiaoqiong, YANG Chunhua, PAN Shaowu, HAN Jie, KUANG Yuxiang, SHI Qingfeng School of Life Science, Beijing Institute of Technology, Beijing 100081, China Abstract Point-of-care testing (POCT) mainly aims at achieving quick and easy medical testing for clinical applications. With advantages of good real-time performance, lower overall costs and independence of expensive equipment, it has been widely used for application areas such as clinical monitoring, inspection and quarantine, home health care and et al. As one of the most important technologies to build POCT instruments, microfl uidic chip is able to fabricate complex system and provide a high testing precision for trace samples. According to different properties of various test objects, the research status of POCT testing technologies based on microfl uidic chip for nucleic acid rest; protein test; cell test was reviewed in this paper. Considering clinical needs of POCT, some basic-research fi ndings of microfl uidic chip for POCT applications were introduced then, which covered microfl uidic chip building techniques, systematization concepts, merchandise design and et al. Finally, research diffi culties and development tendency of POCT mi