改变部分TIR对EPO模拟肽8串联体表达的影响论文.docVIP

　　改变部分TIR对EPO模拟肽8串联体表达的影响论文 .freelHI位点，诱导表达. 结果 DNA测序证明，人工合成的DNA序列已正确插入EPO模拟肽基因8串联体的翻译起始区域.该串联体基因插入pBV220载体后，经42℃诱导.freelimetic peptide;gene multiple repeats Abstract:AIM To observe effect of a synthesized DNA se-quence of translation initiation region（TIR）on expression of8repeats of EOP mimetic peptide gene.METHODS A DNA sequence of translation initiation region imetic peptide gene.The correct rebinant plasmid DNA ed for pBSE-POM8 B.Then8repeats of EPO mimetic peptide gene HI sites of pBV220expression vector.RESULTS DNA sequencing analysis shobent bacterium contained pB-VEPOM8 B olecule m