氩氦刀对T739小鼠LA795皮下移植瘤坏死及细胞凋亡的影响论文.docVIP

　　氩氦刀对T739小鼠LA795皮下移植瘤坏死及细胞凋亡的影响论文 温居一，段蕴铀，邹曰坤，冯华松，聂舟山 【摘要】 目的：研究氩氦刀冷冻消融对T739小鼠LA795肺腺癌皮下移植瘤坏死和细胞凋亡的影响. 评价氩氦刀冷冻治疗非小细胞肺癌的可行性及疗效. 方法：建立T739小鼠LA795肺腺癌皮下移植瘤动物模型. 氩氦刀冷冻后，光学显微镜观察冷冻坏死消融规律；末端标记法(TUNEL)原位检测周边冷冻损伤区带细胞凋亡，免疫组织化学法检查Bc12和Bax表达；ETHODS: Animal model of subcutaneously transplanted tumor of the lung adenocarcinoma LA795 in T739 mice cryoablation,.freelicroscope. Cell apoptosis in peripheral cryoablation zone munohistochemical SABC procedures. The cleavage and activation of Caspase 3 and PARP in peripheral cryoablation zone inated in cell death, sho cryoablation (area of necrotic area/area o