circuitmappingandmanipulation方案.PDFVIP

ORIGINAL RESEARCH ARTICLE NEURAL CIRCUITS published: 10 July 2014 doi: 10.3389/fncir.2014.00076 Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation Julie A. Harris , Karla E. Hirokawa , Staci A. Sorensen , Hong Gu , Maya Mills , Lydia L. Ng , * Phillip Bohn , Marty Mortrud , Benjamin Ouellette , Jolene Kidney , Kimberly A. Smith , Chinh Dang , Susan Sunkin , Amy Bernard , Seung Wook Oh , Linda Madisen and Hongkui Zeng Allen Institute for Brain Science, Seattle, WA, USA Edited by: Significant advances in circuit-level analyses of the brain require tools that allow for Benjamin R. Arenkiel, Baylor College labeling, modulation of gene expression, and monitoring and manipulation of cellular of Medicine, USA activity in specific cell types and/or anatomical regions. Large-scale projects and individual Reviewed by: laboratories have produced hundreds of gene-specific promoter-driven Cre mouse lines Josh Huang, Cold Spring Harbor Laboratory, USA invaluable for enabling genetic access to subpopulations of cells in the brain. However, Petr Tvrdik, University of Utah, USA the potential utility of each line may not be fully realized without systematic whole *Correspondence: brain characterization of transgene expression patterns. We established a high-throughput Julie A. Harris, Allen Institute for in situ hybridization (ISH), imaging and data processing pipeline to describe whole brain Brain Science, 551 N 34th St., ge