“促育生精方”调节环磷酰胺致少弱精症模型大鼠精子特异性钙通道CatSper1、CatSper2的机制研究.pdfVIP

Effects of Cuyushengjingfang on the Expressions of CatSper1 and CatSper2 in Model Rats of Oligoasthenozoospermia Induced by Cyclophosphamide Abstract Objective: To investigate the mechanisms and effects of “Cuyushengjingfang” on CatSper1 and CatSper2 in the sperm of Oligoasthenozoospermia Wistar rats induced by Cyclophosphamide (CP), to provide scientific basis for the clinical applications. Methods: Fifty male Wistar rats were acclimated for six days, then divided randomly into model group(MG), blank control group(BCG), low-dose Cuyushengjing group (LDCG), middle-dose Cuyushengjing group(MDCG) and high-dose Cuyushengjing group(HDCG), 10 rats in each group. Model rats of oligoasthenozoospermia were induced by CP(30mg/kg) injected into the abdominal cavity of rats once a day for 5 days, while BCG were injected with normal saline(NS). The rats in LDCG, MDCG and HDCG were oral administrated with 3ml Cuyushengjingfang for 30 days while the rats in MG and BCG were oral administrated with 3ml NS. The concentration of Cuyushengjingfang in that of LDCG, MDCG and HDCG were 1.73mg/ml, 3.47mg/ml, 6.93mg/ml. Then sperm amount were measured by light microscope. The Ca2+ inner the sperm were detected by floe cytometry. The mRNA and protein expression of CatSper1 and CatSper2 in sperm were measured by PCR and Western blot. Results: Models rats of Oligoasthenozoospermia were created successfully. By observing the numb