低氧通过HIF-1α和HIF-2α上调SKOV3细胞Syndecan-1的表达.docVIP

低氧通过HIF-1α和HIF-2α上调SKOV3细胞Syndecan-1的表达 辛卫娟1，李婷2，赵鑫2，丁岩1，吴乾渝2* 本文档由【中文word文档库】提供，转载分发敬请保留本信息； 中文word文档库免费提供海量范文、教育、学习、政策、报告和经济类word文档。 （1. 复旦大学附属妇产科医院妇产科，上海200032；2. 同济大学附属东方医院妇产科，上海 200120） 摘要 目的：探讨缺氧环境下，卵巢癌细胞株SKOV3中HIF-1α、HIF-2α和Syndecan-1的表达及相互作用关系。方法：利用MatInspector在线分析软件，分析Syndecans转录因子结合位点CoCl2诱导缺氧以及HIF）抑制剂雷帕霉素（Rapamycin）HIF-1α、HIF-2α及Syndecan-1基因水平的表达；Western-blot方法检测HIF-1α、HIF-2α蛋白水平的表达。结果：Syndecan-1的转录因子结合位点中有HIFF（低氧诱导因子家族）/HIF保守序列。缺氧时，HIF-1α mRNA（P0.05）HIF-1α蛋白、HIF-2α mRNA和蛋白Syndecan-1 mRNA水平（P0.05）Rapamycin不能抑制CoCl2模拟缺氧条件下HIF-1α的蛋白表达，但能抑制HIF-2α的表达。Syndecan-1mRNA动态变化曲线和HIF-1α和HIF-2α蛋白水平的动态变化曲线一致低氧可诱导HIF-1α和HIF-2α的表达，α、HIF-2α途径增强Syndecan-1的表达。Rapamycin；HIF-1α；HIF-2α Hypoxia dependent HIF-1α and HIF-2α up regulates the expression of Syndecan-1 in SKOV3 cells XIN Wei-juan1，LI Ting2，ZHAO Xin2，DING Yan1，WU Qian-yu2. 1 Department of Gynecology，Obstetrics and Gynecology Hospital，Fudan University，Shanghai 200032. 2 Department of Gynecology and Obstetrics, East Hospital, Tongji University，Shanghai，200120 【Abstract】Objective：To investigate the expression of hypoxia inducible factor-1α (HIF-1α), hypoxia inducible factor-2α (HIF-2α), and Syndecans-1 , and their relationship during hypoxia in human ovarian cancer cell line SKOV3. Methods：The functions and structures of Syndecan-1 with Matinspector tools. In addition, under hypoxic environments and an exposure to Rapamycin, a suppressor for hypoxia-inducible factors (HIFs), SKOV3 cells were subject to fluorescent quantitative RT-PCR for HIF-1α, HIF-2α and Syndecan-1 genes expressions, to Western-blot for HIF-1α and HIF-2α proteins expressions. Results：There are three HIFF(hypoxia-inducible factor family) /HIF conserved sequences in the Syndecan-1 *通讯作者 Email:xinwj80@126.com transcription factor binding sites. Under the condition of hypoxia, the expressions of HIF-1α mRNA reduced, HIF-1α protein, HIF-2α mRNA and protein increased ( P0.05). Rapamycin couldn’t inhibit the expression of HIF-1α protein, but could inh