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promoter-binding的
Chapter 20;20.1 Introduction20.2 Eukaryotic RNA polymerases consist of many subunits20.3 Promoter elements are defined by mutations and footprinting20.4 RNA polymerase I has a bipartite promoter20.5 RNA polymerase III uses both downstream and upstream promoters20.6 The startpoint for RNA polymerase II20.7 TBP is a universal factor20.8 TBP binds DNA in an unusual way20.9 The basal apparatus assembles at the promoter20.10 Initiation is followed by promoter clearance20.11 A connection between transcription and repair20.12 Promoters for RNA polymerase II have short sequence elements20.13 Some promoter-binding proteins are repressors20.14 Enhancers contain bidirectional elements that assist initiation 20.15 Independent domains bind DNA and activate transcription20.16 The two hybrid assay detects protein-protein interactions20.17 Interaction of upstream factors with the basal apparatus;Enhancer element is a cis-acting sequence that increases the utilization of (some) eukaryotic promoters, and can function in either orientation and in any location (upstream or downstream) relative to the promoter.;Figure 20.1 A typical gene transcribed by RNA polymerase II has a promoter that extends upstream from the site where transcription is initiated. The promoter contains several short (10 bp)sequence elements that bind transcription factors, dispersed over 200 bp. An enhancer containing a more closely packed array of elements that also bind transcription factors may be located several kb distant. (DNA may be coiled or otherwise rearranged so that transcription factors at the promoter and at the enhancer interact to form a large protein complex.);Amanitin (more fully a-amanitin)is a bicyclic octapeptide derived from the poisonous mushroom Amanita phalloides; it inhibits transcription by certain eukaryotic RNA polymerases, especially RNA polymerase II.;Figure 20.2 Eukaryotic RNA polymerase II has 10 subunits. ;Cotransfection is the simultaneous transfection of two mark
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