响应面法优化莴笋废弃物叶绿素提取工艺试验.doc

响应面法优化莴笋废弃物叶绿素提取工艺试验 　　摘要：【目的】莴笋废弃物中含有丰富的天然叶绿素，利用莴笋废弃物提取叶绿素，提高莴笋废弃物利用价值，同时为叶绿素提取原料开拓新的资源。【方法】在单因素试验基础上，选取提取时间、液固比、提取温度、转速为自变量，叶绿素得率为响应值，根据Box-Behnken中心组合试验设计原理采用4因素3水平的响应面分析法，研究各自变量及其交互作用对叶绿素提取量的影响，得到二次多项式回归方程的预测模型。【结果】通过软件分析，本试验回归模型预测莴笋废弃物叶绿素提取的理论最佳工艺条件为：提取时间56.66min，液固比13.71mL/g，提取温度43.35℃，转速152.18rpm，在此条件下，叶绿素理论得率可达0.7706mg/g。验证试验，得到实际叶绿素得率为0.7665mg/g，与理论值相比，其相对误差为0.53%。【结论】最佳提取工艺条件为：提取时间57min，液固比14mL/g，提取温度43℃，转速150rpm。在此条件下叶绿素实际得率为0.7665mg/g，与预测值0.7706mg/g相对误差为0.53% 关键词：莴笋废弃物；叶绿素；提取；响应面法 中图分类号：TS209 文献标识码：A 文章编号：1003-4374（2016）05-0019-06 Abstract：【Objective】The discarded parts of lettuce are rich in natural chlorophyll， and extraction of chlorophyll from lettuce discarded parts can not only beneficial for utilization of waste resources but also play a positive role on environment protection and economic benefit. 【Method】Response surface methodology （RSM） combined with single factor experiments were employed to maximize chlorophyll extraction yield based on Box-Behnken central composite design involving 4 factors （extraction time， liquid/solid ratio， temperature and rotate speed） at 3 levels. The interaction of the respective variables and their influence on the chlorophyll extraction yield were studied， and the simulated quadratic polynomial regression equation of prediction model was set up. 【Result】The results showed that the optimal condition for extraction of chlorophyll was that the extraction time of 57min the liquid/solid ratio of 14mL/g， the extraction temperature of 43℃， and the rotate speed of 150rpm. Under this condition， the measured extraction yield of chlorophyll was 0.7665mg/g， and the relative error was 0.53% compared with the theoretical value of 0.7706mg/g. 【Conclusion】The optimized extraction process parameters can provide a reference for industrial production of chlorophyll from lettuce discarded parts. Key words： lettuce discarded parts； chlorophyll； extraction； Response Surface Methodology 我??是蔬菜大国，随着蔬菜产量提高，蔬菜废弃物的产生也急剧上涨。有研究表