枯草芽孢杆菌利用葡萄糖和木糖生产3-羟基丁酮的分析.pdfVIP

metabolic burden was generated in B. subtilis by overexpression of the pentose phosphate pathway genes. According our previous work, insertional inactivation of genes bdhA, acoA, pta which encoded the enzymes for acetoin degradation and byproduct generation pathways was in favor of improving the yield of the target product acetoin. By introducing the overexpressed xylose transport and heterologous assimilating pathway to the engineered strain BSF3, the optimum strain BSUL13 for acetoin production, was obtained. Under microaerobic conditions, batch cultures in minimal medium with 10 g/L glucose and 5 g/L xylose as the co-existing carbon source showed that the main metabolite was acetoin, and the titer of 5.5 g/L with a yield of 0.70 mol/(mol·sugar), 75% of the theoretical yield was obtained (For 10 g/L glucose and 5 g/L xylose, the theoretical yield is 0.937 mol/(mol·sugar)). This study offered a metabolic engineering strategy on engineering microbe as cell factory for the production of high-valued chemicals from renewable resource. KEY WORDS: Bacillus subtilis, Carbon catabolite repression, Xylose, Acetoin, Metabolic engineering III 目 录 第一章 文献综述 1 1.1 木质纤维素 1 1.1.1 木质纤维素的化学组成及用途 1 1.1.2 木质纤维素的预处理 2 1.2 木糖代谢利用的研究现状 3 1.2.1 木糖的生物转化途径 3 1.2.2 木糖代谢基因工程菌株的构建4 1.2.3 木糖运输途径的改造 7 1.3 枯草芽孢杆菌的木糖代谢研究 8 1.3.1 枯草芽孢杆菌 8 1.3.2 枯草芽孢杆菌的木糖代谢 9 1.3.3 枯草芽孢杆菌的木糖调控机制 9 1.4 葡萄糖阻遏机制 10 1.4.1 细菌的PTS 底物转运系统 11 1.4.2 革兰氏阳性菌的碳代谢阻遏机制 12 1.4.3 革兰氏阴性菌的碳代谢阻遏机制 1