兔肺静脉肌袖心肌细胞动作电位的特性和其某些离子流机制.pdfVIP

生理学报 Acta Physiologica Sinica, April 25, 2006, 58 (2): 129-135 129 研究论文 兔肺静脉肌袖心肌细胞动作电位的特性和一些离子流机制 * 丁怀玉，杨新春 ，刘秀兰，刘泰槰，鲍荣凤 首都医科大学附属北京朝阳医院心脏中心，北京100020 摘要：研究兔肺静脉肌袖心肌细胞(cardiomyocytes from rabbit pulmonary vein sleeves, PVC)动作电位的特性和一些离子流机 制——内向整流钾电流(IK1) 、瞬时外向钾电流(ITo)和非选择性阳离子流(INSCC ) ，并与左心房心肌细胞(left atrial cardiomyocytes, LAC)进行比较。采用全细胞膜片钳技术，记录动作电位和上述各离子流。发现PVC 动作电位时程(action potential duration, APD)较LAC 的明显延长，并可以诱发出第二平台反应。 PVC 上存在INSCC 。PVC 的I K1 、I To 和INSCC 电流密度均较LAC 的 明显减小。PVC 和LAC 存在复极离子流的差异，这种差异构成了两者动作电位差异的基础，进而可能成为肺静脉肌袖致 心律失常特性的重要离子流机制。 关键词：兔；肺静脉；动作电位；离子通道 中图分类号：R 3 2 9 Characteristics of the action potentials and the underlying ionic mechanisms in the cardiomyocytes from rabbit pulmonary vein sleeves * DING Huai-Yu, YANG Xin-Chun , LIU Xiu-Lan, LIU Tai-Feng, BAO Rong-Feng Heart Center, Beijing Chaoyang Hospital Affiliated to the Capital University of Medical Sciences, Beijing 100020, China Abstract: To investigate the characteristics of action potentials and their ionic mechanism in cardiomyocytes from rabbit pulmonary vein sleeves (PVC), and to compare them with those in left atrial cardiomyocytes (LAC), the technique of whole-cell patch clamp was applied. We used current-clamp technique to record action potentials, and voltage-clamp technique to record ionic currents. PVC had longer action potential duration (APD) than LAC, and therefore a second plateau response could be induced easily, suggesting a strong tendency of early afterdepolarization (EAD) genesis in PVC. Non-selective cation current (INSCC ) was first recorded in both LAC and + + + PVC. This I was permeable to K , Na and Cs , sensitive to GdCl but not sensitive to 4-AP. The current densities of inward NSCC 3 rectifier potassium curren