帕金森病大鼠中缝背核5-羟色胺能神经元放电频率与放电形式的变化.pdf

生理学报 Acta Physiologica Sinica , October 25, 2004, 56 (5): 597-602 597 研究论文 帕金森病大鼠中缝背核 5- 羟色胺能神经元放电频率和放电 形式的变化 1 1,2,* 1 3 3 1 褚玉霞 , 刘 健 , 冯 洁 , 王 勇 , 张巧俊 , 李 强 1 西安交通大学医学院生理学教研室; 2 环境与疾病相关基因教育部重点实验室, 西安 710061; 3 西安交通大学第二医院神经内 科, 西安 710004 摘 要: 实验采用玻璃微电极细胞外记录法, 观察了帕金森病(Parkinson’s disease, PD)大鼠中缝背核(dorsal raphe nucleus, DRN) 5- 羟色胺(5-hydroxytrypamine, 5-HT)能神经元电活动的变化。结果发现, 对照组和 PD 组大鼠DRN 中5-HT 能神经元的放电 频率分别为(1.61 ±0.56) Hz 和(2.61 ±1.97) Hz, PD 组大鼠的放电频率显著高于对照组(P0.05) 。在对照组大鼠, 79% 的神经 元呈现规则放电, 21% 为爆发式放电；在PD 组大鼠，具有规则、不规则和爆发式放电的神经元比例分别为 36% 、16% 和 47%, 爆发式放电的 5-HT 能神经元比例明显高于对照组(P0.05) 。结果表明，帕金森病大鼠DRN 中5-HT 能神经元的放电 频率增高, 且爆发式放电增多。 关键词: 帕金森病; 中缝背核; 5- 羟色胺; 6- 羟多巴胺; 电生理学; 大鼠 中图分类号: Q462; R338.2+2 Changes of discharge rate and pattern of 5-hydroxytrypamine neurons of dorsal raphe nucleus in a rat model of Parkinson’s disease 1 1,2,* 1 3 3 1 CHU Yu-Xia , LIU Jian , FENG Jie , WANG Yong , ZHANG Qiao-Jun , Li Qiang 1Department of Physiology, School of Medicine, Xi’an Jiao Tong University; 2Key Laboratory of Enviorment and Genes Related to Diseases, Xi’an 710061, China; 3Department of Neurology, the Second Hospital, Xi’an Jiao Tong University, Xi’an 710004, China Abstract: In the present study, changes of the neuronal activity of 5-hydroxytrypamine (5-HT) neurons of dorsal raphe nucleus(DRN) in a rat model of Parkinson’s disease(PD) were investigated with glass microelectrode recording. The results showed that the discharge rates of 5-HT neurons in control and PD rats were (1.61 ±0.56) Hz and (2.61 ±1.97) Hz, respectively. The discharge rate of PD rats was significantly increased when compared to that of the control rats. In control rats, 79% of 5-HT neurons discharged regularly and 21% in bursts. In PD rats, however, 36% of 5-HT neurons discharged regularly, 16% irregu