点突变试剂盒SMK-101操作流程详解.pdfVIP

08-03 KOD -Plus- Mutagenesis Kit (Code No. SMK-101) 使用说明书 研究用 TOYOBO CO., LTD. Life Science Department OSAKA JAPAN 目 录 [１] 简介 1 [２] 本试剂盒反向PCR突变的流程 2 [３] 制品内容 2 [３] 制品内容 3 [４] 注意事项 3 （１）模板用的质粒DNA 3 （２）PCR引物的设计和要求 4 （３）PCR条件 5 （４）PCR过程中的第二位点突变（目标突变以外的突变） 5 （５）对照质粒 5 [ ５] 操作说明 7 （１）反向PCR 7 （２）用DPN I对模板质粒DNA进行消化 8 （３）PCR产物自身环化 8 （４）转化 8 （５）突变体的确认 9 [６] 疑难解答 10 [７] 参考资料 10 （１）相关培养基和试剂的组成 10 （２）相关产品 11 【 注意 】 本产品为研究用试剂。请勿作为诊断、临床试剂用。 在使用本产品时，请严格遵守实验室的一般注意事项，安全操作。 NOTICE TO PURCHASER: LIMITED LICENSE Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as the patented 5’ Nuclease Process claims in US Patents Nos. 5,210,015 and 5,487,972), no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchasers activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roch