芪白平肺胶囊含药血清介导NO对肺动脉平滑肌细胞KATP通道蛋白表达影响.docVIP

芪白平肺胶囊含药血清介导NO对肺动脉平滑肌细胞KATP通道蛋白表达影响法，体外培养原代大鼠肺动脉平滑肌细胞；Western blot检测不同低氧时间下Kir6.1和SUR2B的表达，以及在一氧化氮合酶抑制剂――Nω硝基L精氨酸甲酯（NωnitroLarginine methyl ester，LNAME）和KATP通道抑制剂――格列本脲（glyburide，GLYB）分别干预下，QBPF对其表达的影响。低氧6 h后Kir6.1和SUR2B蛋白表达开始上调，在低氧24 h达到高峰，低氧48，72 h的蛋白表达出现不同程度下调。在低氧24 h条件下，QBPF能进一步上调KATP通道Kir6.1和SUR2B蛋白表达，且这种上调作用能分别被KATP通道阻断剂GLYB和NO特异性阻断剂LNAME所阻断，提示芪白平肺胶囊具有明确的KATP通道开放作用，其机制可能通过介导NO相关途径上调KATP通道蛋白表达，参与肺血管舒张作用，缓解COPD的发生发展 [关键词] 肺动脉平滑肌细胞； ATP敏感性钾通道； 一氧化氮； 含药血清 Effect of Qibai Pingfei capsule medicated serum on protein expressions of KATP channel in pulmonary arterial smooth muscle cells via nitric oxide ZHU Jie1， LI Zegeng2，3*， WANG Baoqin4， TONG Xiangli4， PENG Qinghe1 （1. School of Integrated Traditional Chinese Western Medicine， Anhui University of Chinese Medicine， Hefei 230038， China； 2. Anhui University of Chinese Medicine， Hefei 230038， China； 3. Institute of Medicine for Respiratory Disease， Anhui Academy of Chinese Medicine， Hefei 230038， China； 4. The First Affiliated Hospital， Anhui University of Chinese Medicine， Hefei 230031， China） [Abstract] To investigate the ATPsensitive potassium channel （KATP channel） protein expressions during different periods under hypoxia condition and explore the effect of Qibai Pingfei capsule medicated serum （hereinafter referred to as QBPF） on the correlation between the protein expressions of KATP channel and nitric oxide in rat pulmonary arterial smooth muscle cells（PASMCs）. Qibai Pingfei capsules were given to SD rats via continuous gavage for 10 days to obtain QBPF. Primary rats PASMCs were cultured by the direct adherent culture method. Western blot was applied to detect the protein expression levels of KATP channel （Kir6.1 and SUR2B） in PASMCs. Then the noncompetitive inhibitor of NO synthase――NωnitroLarginine methyl ester（LNAME） and KATP channel inhibitor――glyburide（GLYB） were applied respectively to evaluate the effect of QBPF on the protein expressions of KATP channel. The protein expression