蛹虫草基质多糖对传染性支气管炎病毒感染鸡免疫指标影响.docVIP

蛹虫草基质多糖对传染性支气管炎病毒感染鸡免疫指标影响 　　摘要：试验应用滴鼻+点眼法对7日龄黄羽肉鸡攻毒建立传染性支气管炎感染模型，采用蛹虫草基质多糖进行治疗试验，通过对肉鸡免疫器官指数、外周血淋巴细胞增殖指数、血清抗体水平和γ-干扰素浓度的检测来研究蛹虫草基质多糖用于防治鸡传染性支气管炎的可能性。结果表明，蛹虫草基质多糖能?虼俳?肉鸡法氏囊和脾脏的生长发育，加快其增重，从而提高免疫器官指数，又能促进外周血淋巴细胞增殖转化水平；同时还能使血清抗体水平和γ-干扰素浓度升高，增强机体的免疫力。因此，蛹虫草基质多糖可作为鸡传染性支气管炎防治的临床候选药物 关键词：蛹虫草基质多糖；传染性支气管炎病毒；免疫指标；临床疗效 中图分类号：S831.7 文献标识码：A 文章编号：0439-8114（2017）07-1298-04 DOI：10.14088/j.cnki.issn0439-8114.2017.07.024 Effects of Polysaccharides from Cordyceps militaris Stroma on Immunity of Chicken Infected by Infectious Bronchitis Virus ZHOU Mei-xian，ZHOU Ye-fei （Department of Food Science， Nanjing Xiaozhuang University， Nanjing 211171， China） Abstract：Seven-day-age broilers artificially infected by infectious bronchitis virus via nasal dripping and eye dropping were used for treatment test. Cordyceps militaris stroma polysaccharides were fed to broilers，and indices of immune organs，indices of lymphocyte stimulating transform，antibody level and interferon-γ concentration were investigated respectively. The results showed that C. militaris stroma polysaccharides could promote growth and development of broiler bursa of fabricius and spleen，and increase indices of immune organs，and also could promote lymphocyte transform causing increased levels of antibody and promote the concentrations of interferon-γ resulting in improving immunity potential. It was conducted that C. militaris stroma polysaccharides could be used for clinical treatment of chicken infected by infectious bronchitis virus. Key words：Cordyceps militaris stroma polysaccharides； infectious bronchitis virus； immune index； clinical treatment 鸡传染性支气管炎（Infectious bronchitis，IB）是由禽传染性支气管炎病毒（Infectious bronchitis virus，IBV）引起的一种急性、高度接触性传染性疾病[1]。近年来，IB对养鸡业（特别是商品肉鸡）的危害日益严重，给养鸡业造成了巨大的经济损失[2]。目前在临床上对该病防制的主要措施是采用接种疫苗的方法，具有显著免疫效果的主要是H120和H52株弱毒疫苗，但由于IBV很容易通过基因突变、缺失、插入和重组发生变异，致使临床上仍经常发生IBV的感染[3] 蛹虫草（Cordyceps militaris）又名北冬虫夏草或北虫草，隶属于子囊菌门麦角菌科虫草属的真菌[4]。目前，市场上销售的蛹虫草大多采用大米、小麦、蚕蛹等培养基进行人工栽培，当蛹虫草子实体被采摘后，大多剩余的