1320nm激光对成纤维细胞增殖和分泌碱性成纤维细胞生长因子及转化生长因子β1的影响.pdfVIP

2 2生1 箍 鲞笠12期 h 』—De—rmatol December 2007，V01．40，N0．12 743 · 论 著 · 1 320 llm激光对成纤维细胞增殖和分泌 碱性成纤维细胞生长因子及 转化生长因子I31的影响 郑振汶 许爱娥 尉晓冬 程浩 【摘要】 目的 探讨非剥脱性1320 nm激光对体外培养的人真皮成纤维细胞增殖和分泌碱性成纤 维细胞生长因子(bFGF)和转化生长因子(TGF一131)的影响。方法 体外培养人真皮成纤维细胞。用1320 m 波长激光15 J／cm ，20 J／cm 和24 J／cm2三个能量分别照射3次。于照射后0、24、48和72 h ELISA法检测 上清液bFGF和TGF一13 1的分泌水平，并计算细胞总数。结果 20 J／cm：以上能量激光照射后成纤维细胞 计数增加，与未照射的对照组比较，差异有统计学意义(P0．05)。激光照射后bFGF分泌的量。在 20 J／cm 和24 J／cm 能量组增加，尤以20 J／cm 为著，24 h达高峰(P0．01)，与对照组比较，差异有统计 学意义。TGF一131分泌的量在照射的各能量组均下降，能量越高，下降越明显，24 h达峰谷，与对照组比较。 高能量组P0．01，其他2个能量组P0．05。结论 1320 nm波长激光使成纤维细胞分裂增殖加速。并 促进bFGF分泌，抑制TGF一131分泌。 【关键词】成纤维细胞；激光；转化生长因子13；成纤维细胞生长因子2 Effects of 1320 nln non-ablative laser on the proliferation of human dermal fibroblasts and the se-- cretion of basic fibroblast growth factor and transform~ growth伍ct0r．Bl 讴NG Zhen．xiao~, XUAi—e’WEJ Xiao—dong,CHENG Hao．Department ofDermatology,Th Peoples Hospital ofHangzhou． Hangzhou 3 J0009,China 【Abstract】Objective To determine the effects of l 320 nlTI non—ablative laser on the proliferation of human dermal fibroblasts，and the secretion of basic fibroblast growth factor (bFGF)and transforming growth factor一13 l (TGF一13 1)in vitro．Methods Human dermal fibroblasts were cultured．and irradiated three times by l 320 nlTI laser at a dose of l 5。20 and 24 J／cm 。respectively．The levels of bFGF and TGF．B l were examined by ELISA at 0，24．48 and 72 h after the irradiation．The number of fibroblasts before and after irradiation were determined．Results The number of fibroblasts and the secretion of bFGF both in— creased after the irradiation at the doses of 20 J／cm0 and 24 J／cm (P0．05)compared with the control cells．Th e effect on bFGF secretion of exl：IOSBle to 20 J／cm2 was more significant than that of exposure to 24 J／cm ：the effect peaked at 24 h after irradiation．The level of TGF．13 l sec