亚硒酸钠对糖尿病肾病大鼠肾脏脂联素表达的影响 - 第二军医大学学报.doc

亚硒酸钠对糖尿病肾病大鼠肾脏脂联素表达的影响 查火兵,魏倩萍* 重庆医科大学附属第一医院老年病科,重庆,400016 [基金项目]：重庆市卫生局课题[07-1-076] [作者介绍]： 查火兵，男， （1980-）， 硕士研究生，主要研究方向糖尿病肾病，电话E-mail：yyjkl76421@126.com [通讯作者]：*魏倩萍，女，硕士研究生导师，博士，TelE-mail：wq163.com The influence of sodium selenite on adiponectin gene expression in kidney of diabetic nephropathy rats ZHA Huo-bing, WEI Qian-ping , Geriatrics Department, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China 【Abstracts】 Objective :To investigate the influence of sodium selenite on adiponectin gene expression in kidney of diabetic nephropathy rats and the relation between sodium selenite and adiponectin , so as to study the role mechanism of sodium selenite and adiponectin in diabetic nephropathy. Methods: Diabetic nephropathy rat models were established by injecting streptozocin intraperitoneally and feeded high fat diet . Three different groups of the rats were employed: control group, diabetic nephropathy group and selenite-treated group.The selenite-treated group taked selenite solution by intragastric administration every day,and other groups taked same dose saline by intragastric administration every day. After 10 weeks, rats were killed, blood and urine sample were got to detect some biochemical indicators. The tablet renal cortex tissue was cut quickly and fixed with 4% glutaral to observe the fine structure by scanning electron microscope. The kidney was cut and made paraffin section to observe pathological changes with light microscope and to locate protein expression with immunohistochemistry analysis. The kidney was cut and the expression of adiponectin mRNA and adiponectin protein were examined through real-time PCR and western blotting. Results: The basic conditions and biochemical indicators of selenite-treated group were better than diabetic nephropathy group, and the same to pathological changes and fine structure. Immunohistochemistry analysis manifested that the coloration of sel