微波消解-原子吸收光谱法测定土壤中的铅和镉 - 济宁医学院学报.doc

微波消解-原子吸收光谱法测定土壤中的铅和镉 王长芹1 张凯1 邓艺2 杜卓群2 (1济宁医学院法医学与医学检验学院，山东272067 2济宁医学院药学院学生，山东 276826) 摘要： 目的 建立微波消解-石墨炉原子吸收光谱法测定土壤中铅和镉含量的方法。方法 利用硝酸-盐酸-氢氟酸作消解液，土壤样品经微波消解，采用石墨炉原子吸收光谱法测定土壤中铅和镉的含量。结果 在优化实验条件下， 铅（2.0～50μg∕L）、镉（0.1～1.0μg∕L）的吸光度（A）与浓度（C）呈良好的线性关系（r值分别为0.9962，0.9971，），检出限分别为1.48μg/L和0.062μg/L，回收率为92.40%～102.6%， RSD为2.63%～5.62%。结论 该方法样品处理效率高、准确性好，用于土壤中铅和镉的测定，结果令人满意。 关键词：微波消解；原子吸收光谱法；土壤；铅；镉 Determination of Lead and Cadmium in Soil Using Microwave Digestion by Atomic Absorption Spectrometry WANG Chang-qin， ZHANG KaiInstitute of Forensic Medicine and Laboratory Medicine，Jining Medical ，Jining，Shandong 272067，ChinaAbstract: Objective To establish a method for the determination of lead and cadmium in soil Using Microwave Digestion by atomic absorption spectrometry (AAS). Methods Using nitric acid -hydrochloric acid -hydrofluoric acid as remove fluid, soil samples by microwave digestion, determination of lead and cadmium content in soil by atomic absorption spectrometry (AAS). Results There was a good linear relationship at the concentration of 2.0~50 μg/L for Pb and at 0.1 ~1.0μg/L for Cd; the correlation coefficient of Pb was 0.9962 and that of Cd was 0.9971.The --------------------------------------------------- 基金项目：2013年济宁市科技局医药卫生科技发展项目（NO.2013jnwk70）；济宁医学院2013年校级科研计划项目（NO.JY2013KJ029）；2012年山东省特色专业“医学检验专业”资助项目（NO.33） 作者简介：王长芹（1979-），女，讲师，从事理化检验研究。 detection limits of lead and cadmium were 1.48μg/L和0.062μg/L, respectively，and the recovery rates were 92.40%～102.6%, the RSDs were 2.63%～5.62%. Conclusion The method was efficient for sample reatment and accurate. The method was employed for measurement of Pb and Cd in soil, and satisfactory results were obtained. Keywords：Microwave digestion；Atomic absorption spectrometry；Soil；Lead；Cadmium ,用少量超纯水洗涤消解罐，合并洗涤液于坩埚中，将坩埚置电热板上，加热赶酸至大约2ml，冷却后将溶液转移至25ml比色管中，用0.5%硝酸溶液洗涤坩埚，合并洗液，用80 g/L 磷酸二氢铵溶液作基体改进剂，并用0.5%硝酸溶液定容至刻度，同时做空白试验。微波消解的工作条件见表1。 表1 微波消解工作条件 消解程序 温度/℃ 时间/min 第1 阶段 90