层析柱(国外英语资料).doc

层析柱 Chromatography column is the main body of gel chromatography, usually using glass tube or plexiglass tube. The diameter of the chromatographic column does not affect the separation degree, and the amount of the sample is large, and the diameter of the column can be increased. The diameter of the prepared gel column is more than 2 cm, but the sample shall be uniformly distributed on the gel column surface when adding the sample. In addition, the diameter increases, the elution liquid volume increases, the sample dilution is big. The degree of separation depends on the column height. In order to separate the different components, the gel column must have a proper height, and the separation is related to the square root of the column height. But the soft gel column is too high and the extrusion deformation is blocked, generally not more than 1 meters. When divided into groups, a short column, a common gel column, is 20-30 cm long, and the column height is compared with the diameter of 5:1 - 10:1. The volume of the gel bed is 4-10 times of the volume of the sample solution. The line of column height and diameter at grade separation is 20:1 - 100:1, and the common gel column is 50 * 25 cm, 10 * 25 cm. The dead volume of the column under the filter plate should be as small as possible, if the palmar branch of the dead volume of the filter plate, is separated between the possibility of re mixing is large, the result is the effect of elution peak and trailing like, lower resolution. In accurate separation, the dead volume must not exceed 1/1000 of the total bed volume. In column chromatography separation and extraction of oridonin as an example Medium pressure separation chromatography column operation method In column chromatography separation and extraction of oridonin as an example 1 treatment of adsorbent and column loading A certain amount of thin layer chromatography silica gel loaded enamel plate placed in an oven to 105. After C activation, 2H can be removed an